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Interplay between R513 methylation and S516 phosphorylation of the cardiac voltage-gated sodium channel

Arginine methylation is a novel post-translational modification within the voltage-gated ion channel superfamily, including the cardiac sodium channel, Nav1.5. We show that Nav1.5 R513 methylation decreases S516 phosphorylation rate by 4 orders of magnitude, the first evidence of protein kinase A inhibition by arginine methylation. Reciprocally, S516 phosphorylation blocks R513 methylation. Nav1.5 p.G514C, associated to cardiac conduction disease, abrogates R513 methylation, while leaving S516 phosphorylation rate unchanged. This is the first report of methylation-phosphorylation cross-talk of a cardiac ion channel

Springer Verlag

Manager: Ministerio de Ciencia e Innovación (Espanya)
Author: Beltrán Álvarez, Pedro
Feixas Geronès, Ferran
Osuna Oliveras, Sílvia
Díaz Hernández, Rubí
Brugada, Ramon
Pagans i Lista, Sara
Date: 2015
Abstract: Arginine methylation is a novel post-translational modification within the voltage-gated ion channel superfamily, including the cardiac sodium channel, Nav1.5. We show that Nav1.5 R513 methylation decreases S516 phosphorylation rate by 4 orders of magnitude, the first evidence of protein kinase A inhibition by arginine methylation. Reciprocally, S516 phosphorylation blocks R513 methylation. Nav1.5 p.G514C, associated to cardiac conduction disease, abrogates R513 methylation, while leaving S516 phosphorylation rate unchanged. This is the first report of methylation-phosphorylation cross-talk of a cardiac ion channel
Format: application/pdf
Document access: http://hdl.handle.net/10256/10361
Language: eng
Publisher: Springer Verlag
Collection: info:eu-repo/semantics/altIdentifier/doi/10.1007/s00726-014-1890-0
info:eu-repo/semantics/altIdentifier/issn/0939-4451
info:eu-repo/semantics/altIdentifier/eissn/1438-2199
info:eu-repo/grantAgreement/MICINN//CTQ2011-25086/ES/MODELIZACION MULTIESCALAR EN (BIO)QUIMICA/
info:eu-repo/grantAgreement/MICINN//SAF2011-27627/ES/ESTUDIO DE LOS MECANISMOS MOLECULARES QUE REGULAN LA EXPRESION Y FUNCION DE SCN5A/
info:eu-repo/grantAgreement/EC/FP7/630978/EU/Computational Exploration of Directed Evolution rules for tuning enzymatic activities/DIREVENZYME
Rights: Tots els drets reservats
Subject: Sodium channel
Arginina -- Metilació
Arginine -- Methylation
Fosforilació
Phosphorylation
Title: Interplay between R513 methylation and S516 phosphorylation of the cardiac voltage-gated sodium channel
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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