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The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids

The present study provides new insights on the role of the potato (Solanum tuberosum) suberin feruloyl transferase FHT in native and wound tissues, leading to conclusions about hitherto unknown properties of the phellogen. In agreement with the enzymatic role of FHT, it is shown that its transcriptional activation and protein accumulation are specific to tissues that undergo suberization such as the root boundary layers of the exodermis and the endodermis, along with the tuber periderm. Remarkably, FHT expression and protein accumulation within the periderm is restricted to the phellogen derivative cells with phellem identity. FHT levels in the periderm are at their peak near harvest during periderm maturation, with the phellogen becoming meristematically inactive and declining thereafter. However, periderm FHT levels remain high for several months after harvest, suggesting that the inactive phellogen retains the capacity to synthesize ferulate esters. Tissue wounding induces FHT expression and the protein accumulates from the first stages of the healing process onwards. FHT is up-regulated by abscisic acid and down-regulated by salicylic acid, emphasizing the complex regulation of suberin synthesis and wound healing. These findings open up new prospects important for the clarification of the suberization process and yield important information with regard to the skin quality of potatoes

The authors thank Professor S. Prat (Centro Nacional de Biotecnologia, Madrid) and Dr E. Dominguez (Centre de Recerca en Agrigenomica CRAG, Barcelona) for providing potato ssp. andigena, for support, and for helpful experimental advice; Dr P. Suarez (CRAG) for kindly lending us the ultracentrifuge; Professor D. Ludevid, Dr S. Irar, and Dr M. P. Gonzalez (CRAG), and Dr T. Roscoe (Institut de Recherche pour le Development, Montpellier) for fruitful suggestions regarding protein and antibody production, immunolocalization, and GUS staining; and Dr J. Castro (Biology Department, University of Girona, UdG) for helpful advice on setting up the western blot conditions. We also thank Mr J. Blavia and D. Reyes (Serveis Tecnics de Recerca, UdG) and S. Gomez (Departament de Biologia, UdG) for their valuable assistance in carrying out the laboratory work. This work was supported by the Ministerio de Innovacion y Ciencia [AGL2009-13745], the Ministerio de Educacion y Ciencia [FPI grant to PB], and the Ministerio de Economia y Competitividad [AGL2012-36725]

Journal of Experimental Botany, 2013, vol. 64, núm. 11, p. 3225-3236

Oxford University Press (OUP)

Manager: Ministerio de Economía y Competitividad (Espanya)
Ministerio de Ciencia e Innovación (Espanya)
Author: Boher Genís, Pau
Serra, Olga
Soler, Marçal
Molinas de Ferrer, Maria Lluïsa
Figueras i Vall-llosera, Mercè
Date: 2013
Abstract: The present study provides new insights on the role of the potato (Solanum tuberosum) suberin feruloyl transferase FHT in native and wound tissues, leading to conclusions about hitherto unknown properties of the phellogen. In agreement with the enzymatic role of FHT, it is shown that its transcriptional activation and protein accumulation are specific to tissues that undergo suberization such as the root boundary layers of the exodermis and the endodermis, along with the tuber periderm. Remarkably, FHT expression and protein accumulation within the periderm is restricted to the phellogen derivative cells with phellem identity. FHT levels in the periderm are at their peak near harvest during periderm maturation, with the phellogen becoming meristematically inactive and declining thereafter. However, periderm FHT levels remain high for several months after harvest, suggesting that the inactive phellogen retains the capacity to synthesize ferulate esters. Tissue wounding induces FHT expression and the protein accumulates from the first stages of the healing process onwards. FHT is up-regulated by abscisic acid and down-regulated by salicylic acid, emphasizing the complex regulation of suberin synthesis and wound healing. These findings open up new prospects important for the clarification of the suberization process and yield important information with regard to the skin quality of potatoes
The authors thank Professor S. Prat (Centro Nacional de Biotecnologia, Madrid) and Dr E. Dominguez (Centre de Recerca en Agrigenomica CRAG, Barcelona) for providing potato ssp. andigena, for support, and for helpful experimental advice; Dr P. Suarez (CRAG) for kindly lending us the ultracentrifuge; Professor D. Ludevid, Dr S. Irar, and Dr M. P. Gonzalez (CRAG), and Dr T. Roscoe (Institut de Recherche pour le Development, Montpellier) for fruitful suggestions regarding protein and antibody production, immunolocalization, and GUS staining; and Dr J. Castro (Biology Department, University of Girona, UdG) for helpful advice on setting up the western blot conditions. We also thank Mr J. Blavia and D. Reyes (Serveis Tecnics de Recerca, UdG) and S. Gomez (Departament de Biologia, UdG) for their valuable assistance in carrying out the laboratory work. This work was supported by the Ministerio de Innovacion y Ciencia [AGL2009-13745], the Ministerio de Educacion y Ciencia [FPI grant to PB], and the Ministerio de Economia y Competitividad [AGL2012-36725]
Format: application/pdf
Citation: 019467
ISSN: 0022-0957 (versió paper)
1460-2431 (versió electrònica)
Document access: http://hdl.handle.net/10256/10916
Language: eng
Publisher: Oxford University Press (OUP)
Collection: MINECO/PN 2013-2015/AGL2012-36725
MICINN/PN 2010-2012/AGL2009-13745
Reproducció digital del document publicat a: http://dx.doi.org/10.1093/jxb/ert163
Articles publicats (D-B)
Is part of: Journal of Experimental Botany, 2013, vol. 64, núm. 11, p. 3225-3236
Rights: Attribution-NonCommercial 3.0 Spain
Rights URI: http://creativecommons.org/licenses/by-nc/3.0/es/
Subject: Biosíntesi
Biosynthesis
Title: The potato suberin feruloyl transferase FHT which accumulates in the phellogen is induced by wounding and regulated by abscisic and salicylic acids
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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