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Specific archaeal communities are selected on the root surfaces of ruppia spp. and phragmites australis

Archaea, although being active members of microbial communities, have been poorly characterized in the rhizoplane of wetland plants. In this study, we compared the archaeal communities found on the root surface of Ruppia spp. and Phragmites australis. Four lagoons, two euhaline andtwo oligohaline, were sampled during two periods of the year.Archaeal communities were investigated by means of 16SrRNA gene cloning and sequencing. Archaeal sequencesclustered in 171 distinct Operational Taxonomic Units affiliatedto the Euryarchaeota (43 %), Crenarchaeota (36 %) andThaumarchaeota (21 %). Communities found in theunvegetated sediment samples showed a high variabilitymainly due to changes in the pH and conductivity. Ruppiaspp. roots exhibited a clear selection effect overHalobacteriales (>80 % of detected sequences) despite thgeographical location and sampling time. In contrast, thearchaeal community on the root surface of Phragmitesaustralis was largely dependent on the sampling locationand time. Methanogenic lineages were not found to be relevantin almost any of the analysed samples. Our findingssuggest that archaeal communities in the root surface of thestudied macrophytes are mainly composed (40 to 86 %) ofputative heterotrophic archaea, such as Halobacteriales,Thermoplasmatales and the Miscellaneous CrenarchaeoticGroup

This work was financially supported by the Spanish Ministerio de Ciencia e Innovacion through projects ARCANOX (CGL2009-13318) and MACROBIOTECH (CGL2011-23907). Authors are grateful to the Do ana Biological Station for technical support (ICTS 24/2007) and to Dr. X. Quintana and Dr. R. Lopez-Flores for sampling

© Wetlands, 2014, vol. 34, núm. 2, p. 403-411

Springer Verlag

Author: Llirós Dupré, Marc
Trias Mansilla, Rosalia
Borrego i Moré, Carles
Bañeras Vives, Lluís
Date: 2014
Abstract: Archaea, although being active members of microbial communities, have been poorly characterized in the rhizoplane of wetland plants. In this study, we compared the archaeal communities found on the root surface of Ruppia spp. and Phragmites australis. Four lagoons, two euhaline andtwo oligohaline, were sampled during two periods of the year.Archaeal communities were investigated by means of 16SrRNA gene cloning and sequencing. Archaeal sequencesclustered in 171 distinct Operational Taxonomic Units affiliatedto the Euryarchaeota (43 %), Crenarchaeota (36 %) andThaumarchaeota (21 %). Communities found in theunvegetated sediment samples showed a high variabilitymainly due to changes in the pH and conductivity. Ruppiaspp. roots exhibited a clear selection effect overHalobacteriales (>80 % of detected sequences) despite thgeographical location and sampling time. In contrast, thearchaeal community on the root surface of Phragmitesaustralis was largely dependent on the sampling locationand time. Methanogenic lineages were not found to be relevantin almost any of the analysed samples. Our findingssuggest that archaeal communities in the root surface of thestudied macrophytes are mainly composed (40 to 86 %) ofputative heterotrophic archaea, such as Halobacteriales,Thermoplasmatales and the Miscellaneous CrenarchaeoticGroup
This work was financially supported by the Spanish Ministerio de Ciencia e Innovacion through projects ARCANOX (CGL2009-13318) and MACROBIOTECH (CGL2011-23907). Authors are grateful to the Do ana Biological Station for technical support (ICTS 24/2007) and to Dr. X. Quintana and Dr. R. Lopez-Flores for sampling
Format: application/pdf
ISSN: 0277-5212 (versió paper)
1943-6246 (versió electrònica)
Document access: http://hdl.handle.net/10256/11306
Language: eng
Publisher: Springer Verlag
Collection: MICINN/PN 2012-2015/CGL2011-23907
MICINN/PN 2010-2012/CGL2009-13318
Reproducció digital del document publicat a: http://dx.doi.org/10.1007/s13157-013-0507-9
Articles publicats (D-B)
Is part of: © Wetlands, 2014, vol. 34, núm. 2, p. 403-411
Rights: Tots els drets reservats
Subject: Zones humides
Wetlands
Plantes aquàtiques
Aquatic plants
Title: Specific archaeal communities are selected on the root surfaces of ruppia spp. and phragmites australis
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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