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Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems

Denitrifying bioelectrochemical systems (d-BES) are a promising technology for nitrate removal from wastewaters. Microbial community monitoring is required to pave the way to application. In this study, for the first time flow cytometry combined with molecular biology techniques is exploited to monitor and determine the structure-function relationship of the microbiome of a denitrifying biocathode. Stable cathode performance at poised potential (-0.32 V vs. Ag/AgCl) was monitored, and different stress-tests were applied (reactor leakage, nitrate concentration, buffer capacity). Stress-tests shifted the reactor microbiome and performance. The monitoring campaign covered a wide range of nitrate consumption rates (from 15 to 157 mg N L-NCC(-1) d(-1)), current densities (from 0 to 25 mA L-NCC(-1)) and denitrification intermediates (nitrite and nitrous oxide consumption rates varied from 0 to 56 mg N L-NCC(-1) d(-1)). The reactor microbiome (composed of 21 subcommunities) was characterized and its structure-function relationship was revealed. A key role for Thiobacillus sp. in the bioelectrochemical reduction of nitrate was suggested, while a wider number of subcommunities were involved in NO2- and N2O reduction. It was demonstrated that different bacteria catalyze each denitrification step in a biocathode. This study contributed significantly to understanding denitrifying biocathodes, paving the way for their knowledge-driven engineering

This work was supported by the Spanish Government (CTQ2011-23632, CTQ2014-SGR-1168) and the Helmholtz-Association within the Research Programme Terrestrial Environment. F.H. acknowledges support by the BMBF (Research Award "Next generation biotechnological processes - Biotechnology 2020+") and the Helmholtz-Association (Young Investigators Group). N.P. was supported by the Catalan Government (2012FI-B00941) and the University of Girona (MOB2014-ref22)

© RSC Advances, 2015, vol. 5, núm. 84, p. 68326-68333

Royal Society of Chemistry (RSC)

Manager: Ministerio de Ciencia e Innovación (Espanya)
Generalitat de Catalunya. Agència de Gestió d’Ajuts Universitaris i de Recerca
Author: Pous Rodríguez, Narcís
Koch, Christin
Vilà-Rovira, Albert
Balaguer i Condom, Maria Dolors
Colprim Galceran, Jesús
Muehlenberg, Jana
Mueller, Simone
Harnisch, Falk
Puig Broch, Sebastià
Date: 2015
Abstract: Denitrifying bioelectrochemical systems (d-BES) are a promising technology for nitrate removal from wastewaters. Microbial community monitoring is required to pave the way to application. In this study, for the first time flow cytometry combined with molecular biology techniques is exploited to monitor and determine the structure-function relationship of the microbiome of a denitrifying biocathode. Stable cathode performance at poised potential (-0.32 V vs. Ag/AgCl) was monitored, and different stress-tests were applied (reactor leakage, nitrate concentration, buffer capacity). Stress-tests shifted the reactor microbiome and performance. The monitoring campaign covered a wide range of nitrate consumption rates (from 15 to 157 mg N L-NCC(-1) d(-1)), current densities (from 0 to 25 mA L-NCC(-1)) and denitrification intermediates (nitrite and nitrous oxide consumption rates varied from 0 to 56 mg N L-NCC(-1) d(-1)). The reactor microbiome (composed of 21 subcommunities) was characterized and its structure-function relationship was revealed. A key role for Thiobacillus sp. in the bioelectrochemical reduction of nitrate was suggested, while a wider number of subcommunities were involved in NO2- and N2O reduction. It was demonstrated that different bacteria catalyze each denitrification step in a biocathode. This study contributed significantly to understanding denitrifying biocathodes, paving the way for their knowledge-driven engineering
This work was supported by the Spanish Government (CTQ2011-23632, CTQ2014-SGR-1168) and the Helmholtz-Association within the Research Programme Terrestrial Environment. F.H. acknowledges support by the BMBF (Research Award "Next generation biotechnological processes - Biotechnology 2020+") and the Helmholtz-Association (Young Investigators Group). N.P. was supported by the Catalan Government (2012FI-B00941) and the University of Girona (MOB2014-ref22)
Format: application/pdf
Citation: 023557
ISSN: 2046-2069
Document access: http://hdl.handle.net/10256/11527
Language: eng
Publisher: Royal Society of Chemistry (RSC)
Collection: MICINN/PN 2012-2014/CTQ2011-23632
AGAUR/2014-2016/2014 SGR 1168
AGAUR/2012 FI-B 00941
Reproducció digital del document publicat a: http://dx.doi.org/10.1039/c5ra12113b
Articles publicats (D-EQATA)
Is part of: © RSC Advances, 2015, vol. 5, núm. 84, p. 68326-68333
Rights: Tots els drets reservats
Subject: Desnitrificació
Denitrification
Bioelectroquímica
Bioelectrochemistry
Aigües residuals -- Depuració -- Desnitrificació
Sewage -- Purification -- Nitrogen removal
Title: Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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