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Shifts in microbial community structure and function in light- and dark-grown biofilms driven by warming

Biofilms are dynamic players in biogeochemical cycling in running waters and are subjected to environmental stressors like those provoked by climate change. We investigated whether a 2 degrees C increase in flowing water would affect prokaryotic community composition and heterotrophic metabolic activities of biofilms grown under light or dark conditions. Neither light nor temperature treatments were relevant for selecting a specific bacterial community at initial phases (7-day-old biofilms), but both variables affected the composition and function of mature biofilms (28-day-old). In dark-grown biofilms, changes in the prokaryotic community composition due to warming were mainly related to rotifer grazing, but no significant changes were observed in functional fingerprints. In light-grown biofilms, warming also affected protozoan densities, but its effect on prokaryotic density and composition was less evident. In contrast, heterotrophic metabolic activities in light-grown biofilms under warming showed a decrease in the functional diversity towards a specialized use of several carbohydrates. Results suggest that prokaryotes are functionally redundant in dark biofilms but functionally plastic in light biofilms. The more complex and self-serving light-grown biofilm determines a more buffered response to temperature than dark-grown biofilms. Despite the moderate increase in temperature of only 2 degrees C, warming conditions drive significant changes in freshwater biofilms, which responded by finely tuning a complex network of interactions among microbial populations within the biofilm matri

This study was funded by the Spanish Ministry of Economy and Competitiveness, project FLUMED-HOTSPOTS (CGL2011-30151-C02-01). V. Diaz Villanueva participated in this project supported by FONCYT (Argentina) (PICT-2007-01747) and CONICET (PIP 114-201101-00304)

© Environmental Microbiology, 2014, vol. 16, núm. 8, p. 2550-2567

Wiley

Author: Romaní i Cornet, Anna M.
Borrego i Moré, Carles
Díaz Villanueva, Verónica
Freixa Casals, Anna
Gich Batlle, Frederic
Ylla i Monfort, Irene
Date: 2014
Abstract: Biofilms are dynamic players in biogeochemical cycling in running waters and are subjected to environmental stressors like those provoked by climate change. We investigated whether a 2 degrees C increase in flowing water would affect prokaryotic community composition and heterotrophic metabolic activities of biofilms grown under light or dark conditions. Neither light nor temperature treatments were relevant for selecting a specific bacterial community at initial phases (7-day-old biofilms), but both variables affected the composition and function of mature biofilms (28-day-old). In dark-grown biofilms, changes in the prokaryotic community composition due to warming were mainly related to rotifer grazing, but no significant changes were observed in functional fingerprints. In light-grown biofilms, warming also affected protozoan densities, but its effect on prokaryotic density and composition was less evident. In contrast, heterotrophic metabolic activities in light-grown biofilms under warming showed a decrease in the functional diversity towards a specialized use of several carbohydrates. Results suggest that prokaryotes are functionally redundant in dark biofilms but functionally plastic in light biofilms. The more complex and self-serving light-grown biofilm determines a more buffered response to temperature than dark-grown biofilms. Despite the moderate increase in temperature of only 2 degrees C, warming conditions drive significant changes in freshwater biofilms, which responded by finely tuning a complex network of interactions among microbial populations within the biofilm matri
This study was funded by the Spanish Ministry of Economy and Competitiveness, project FLUMED-HOTSPOTS (CGL2011-30151-C02-01). V. Diaz Villanueva participated in this project supported by FONCYT (Argentina) (PICT-2007-01747) and CONICET (PIP 114-201101-00304)
Format: application/pdf
ISSN: 1462-2912 (versió paper)
1462-2920 (versió electrònica)
Document access: http://hdl.handle.net/10256/11549
Language: eng
Publisher: Wiley
Collection: MICINN/PN 2012-2014/CGL2011-30151-C02-01
Reproducció digital del document publicat a: http://dx.doi.org/10.1111/1462-2920.12428
Articles publicats (D-CCAA)
Is part of: © Environmental Microbiology, 2014, vol. 16, núm. 8, p. 2550-2567
Rights: Tots els drets reservats
Subject: Biofilms
Ecologia microbiana
Microbial ecology
Title: Shifts in microbial community structure and function in light- and dark-grown biofilms driven by warming
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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