Ítem


The production of recombinant cationic α-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum

Synthetic linear antimicrobial peptides with cationic α-helical structures, such as BP100, are valuable as novel therapeutics and preservatives. However, they tend to be toxic when expressed at high levels as recombinant peptides in plants, and they can be difficult to detect and isolate from complex plant tissues because they are strongly cationic and display low extinction coefficient and extremely limited immunogenicity. We therefore expressed BP100 with a C-terminal tag which preserved its antimicrobial activity and demonstrated significant accumulation in plant cells. We used a fluorescent tag to trace BP100 following transiently expression in Nicotiana benthamiana leaves and showed that it accumulated in large vesicles derived from the endoplasmic reticulum (ER) along with typical ER luminal proteins. Interestingly, the formation of these vesicles was induced by BP100. Similar vesicles formed in stably transformed Arabidopsis thaliana seedlings, but the recombinant peptide was toxic to the host during latter developmental stages. This was avoided by selecting active BP100 derivatives based on their low haemolytic activity even though the selected peptides remained toxic to plant cells when applied exogenously at high doses. Using this strategy, we generated transgenic rice lines producing active BP100 derivatives with a yield of up to 0.5% total soluble protein

This work was supported by the Spanish Ministerio de Ciencia e Innovacion (projects AGL2010-17181/AGR and PLANT-KBBE EUI2008-03769) and BMBF PLANT-KBBE FKZ0315456B. We thank J. Messeguer and E. Mele (IRTA, Cabrils) for valuable suggestions; D. Ludevid (CRAG, Barcelona) for providing plasmid material; M. Amenos (CRAG) and the staff of the Microscopy Service (Universitat Autonoma de Barcelona) for technical assistance; E. Badosa and L. Montesinos for assessing the antimicrobial activity of chemically synthesized peptides; and the scientific writer R. M. Twyman for revision of the manuscript

Wiley

Director: Ministerio de Ciencia e Innovación (Espanya)
Autor: Company Casadevall, Núria
Nadal i Matamala, Anna
La Paz Gallego, José Luís
Martínez, Sílvia
Rasche, Stefan
Schillberg, Stefan
Montesinos Seguí, Emilio
Pla i de Solà-Morales, Maria
Data: 2014
Resum: Synthetic linear antimicrobial peptides with cationic α-helical structures, such as BP100, are valuable as novel therapeutics and preservatives. However, they tend to be toxic when expressed at high levels as recombinant peptides in plants, and they can be difficult to detect and isolate from complex plant tissues because they are strongly cationic and display low extinction coefficient and extremely limited immunogenicity. We therefore expressed BP100 with a C-terminal tag which preserved its antimicrobial activity and demonstrated significant accumulation in plant cells. We used a fluorescent tag to trace BP100 following transiently expression in Nicotiana benthamiana leaves and showed that it accumulated in large vesicles derived from the endoplasmic reticulum (ER) along with typical ER luminal proteins. Interestingly, the formation of these vesicles was induced by BP100. Similar vesicles formed in stably transformed Arabidopsis thaliana seedlings, but the recombinant peptide was toxic to the host during latter developmental stages. This was avoided by selecting active BP100 derivatives based on their low haemolytic activity even though the selected peptides remained toxic to plant cells when applied exogenously at high doses. Using this strategy, we generated transgenic rice lines producing active BP100 derivatives with a yield of up to 0.5% total soluble protein
This work was supported by the Spanish Ministerio de Ciencia e Innovacion (projects AGL2010-17181/AGR and PLANT-KBBE EUI2008-03769) and BMBF PLANT-KBBE FKZ0315456B. We thank J. Messeguer and E. Mele (IRTA, Cabrils) for valuable suggestions; D. Ludevid (CRAG, Barcelona) for providing plasmid material; M. Amenos (CRAG) and the staff of the Microscopy Service (Universitat Autonoma de Barcelona) for technical assistance; E. Badosa and L. Montesinos for assessing the antimicrobial activity of chemically synthesized peptides; and the scientific writer R. M. Twyman for revision of the manuscript
Format: application/pdf
Cita: 24102775
Accés al document: http://hdl.handle.net/10256/11698
Llenguatge: eng
Editor: Wiley
Col·lecció: info:eu-repo/semantics/altIdentifier/doi/10.1111/pbi.12119
info:eu-repo/semantics/altIdentifier/issn/1467-7644
info:eu-repo/semantics/altIdentifier/eissn/1467-7652
info:eu-repo/grantAgreement/MICINN//AGL2010-17181/ES/EFECTOS NO INTENCIONADOS DE TRANSGENES Y OPTIMIZACION DE LA PRODUCCION DE PEPTIDOS ANTIMICROBIANOS DE USO FITOSANITARIO EN PLANTAS-BIOFACTORIA/
Drets: Tots els drets reservats
Matèria: Transgenic plant
Antibiòtics pèptids
Peptide antibiotics
Farmacologia molecular
Molecular pharmacology
Plantes transgèniques
Transgenic plants
Bacteris fitopatògens
Phytopathogenic bacteria
Plantes -- Malalties
Plant diseases
Títol: The production of recombinant cationic α-helical antimicrobial peptides in plant cells induces the formation of protein bodies derived from the endoplasmic reticulum
Tipus: info:eu-repo/semantics/article
Repositori: DUGiDocs

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