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| Ministerio de Ciencia e Innovación (Espanya) | |
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Serrano, Soraya
CallÃs Figueres, Mariona Vilanova i Brugués, Maria Benito i Mundet, Antoni Laurents, Douglas V. Ribó i Panosa, Marc Bruix, Marta |
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Onconase® FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase® sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase’s® active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR 1H, 13C and 15N resonance chemical shift assignments at pH 5.2 and 35 C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase ® is conserved in the FL-G zymogen This work was supported by the projects, CTQ2008-0080, CTQ2010-21567-C02-02 and BFU2009-06935/ BMC from MICINN and PUG2008A from the Universitat de Girona |
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| application/pdf | |
| http://hdl.handle.net/10256/11967 | |
| eng | |
| Springer Verlag | |
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s12104-012-9367-0 info:eu-repo/semantics/altIdentifier/issn/1874-2718 info:eu-repo/semantics/altIdentifier/eissn/1874-270X info:eu-repo/grantAgreement/MICINN//BFU2009-06935/ES/Bases Moleculares Del Plegamiento Y Citotoxicidad De Las Ribonucleasas Pancreaticas. Evaluacion De La Actividad Citotoxica Y Diseño De Estrategias Para Su Control Mediante Splicing Proteico./ |
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| Tots els drets reservats | |
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Enginyeria de proteïnes
Protein engineering Enzims Enzymes |
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| 1H, 13C and 15N resonance assignments of the Onconase FL-G zymogen | |
| info:eu-repo/semantics/article | |
| DUGiDocs |
