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Cultured representatives of two major phylogroups of human colonic Faecalibacterium prausnitzii can utilize pectin, uronic acids, and host-derived substrates for growth

Faecalibacterium prausnitzii is one of the most abundant commensal bacteria in the healthy human large intestine, but information on genetic diversity and substrate utilization is limited. Here, we examine the phylogeny, phenotypic characteristics, and influence of gut environmental factors on growth of F. prausnitzii strains isolated from healthy subjects. Phylogenetic analysis based on the 16S rRNA sequences indicated that the cultured strains were representative of F. prausnitzii sequences detected by direct analysis of fecal DNA and separated the available isolates into two phylogroups. Most F. prausnitzii strains tested grew well under anaerobic conditions on apple pectin. Furthermore, F. prausnitzii strains competed successfully in coculture with two other abundant pectin-utilizing species, Bacteroides thetaiotaomicron and Eubacterium eligens, with apple pectin as substrate, suggesting that this species makes a contribution to pectin fermentation in the colon. Many F. prausnitzii isolates were able to utilize uronic acids for growth, an ability previously thought to be confined to Bacteroides spp. among human colonic anaerobes. Most strains grew on N-acetylglucosamine, demonstrating an ability to utilize host-derived substrates. All strains tested were bile sensitive, showing at least 80% growth inhibition in the presence of 0.5μg/ml bile salts, while inhibition at mildly acidic pH was strain dependent. These attributes help to explain the abundance of F. prausnitzii in the colonic community but also suggest factors in the gut environment that may limit its distribution

We acknowledge the Scottish Government Rural Environment Research and Analysis Directorate for support. Mireia Lopez-Siles was awarded a fellowship from the Spanish Ministerio de Educación to support a research stay at the RINH and is also the recipient of an FI grant from the Generalitat de Catalunya (2010FI_B2 00135), which receives support from the European Union Commissionate. This work was partially funded by the Spanish Ministry of Education and Science through project SAF2006-00414

© Applied and Environmental Microbiology, 2012, vol. 78, p. 420-428

American Society for Microbiology

Author: Flint, Harry J.
López Siles, Mireia
Khan, Tanweer M
Duncan, Sylvia H.
Harmsen, Hermie J M
Garcia-Gil, L. J.
Date: 2012
Abstract: Faecalibacterium prausnitzii is one of the most abundant commensal bacteria in the healthy human large intestine, but information on genetic diversity and substrate utilization is limited. Here, we examine the phylogeny, phenotypic characteristics, and influence of gut environmental factors on growth of F. prausnitzii strains isolated from healthy subjects. Phylogenetic analysis based on the 16S rRNA sequences indicated that the cultured strains were representative of F. prausnitzii sequences detected by direct analysis of fecal DNA and separated the available isolates into two phylogroups. Most F. prausnitzii strains tested grew well under anaerobic conditions on apple pectin. Furthermore, F. prausnitzii strains competed successfully in coculture with two other abundant pectin-utilizing species, Bacteroides thetaiotaomicron and Eubacterium eligens, with apple pectin as substrate, suggesting that this species makes a contribution to pectin fermentation in the colon. Many F. prausnitzii isolates were able to utilize uronic acids for growth, an ability previously thought to be confined to Bacteroides spp. among human colonic anaerobes. Most strains grew on N-acetylglucosamine, demonstrating an ability to utilize host-derived substrates. All strains tested were bile sensitive, showing at least 80% growth inhibition in the presence of 0.5μg/ml bile salts, while inhibition at mildly acidic pH was strain dependent. These attributes help to explain the abundance of F. prausnitzii in the colonic community but also suggest factors in the gut environment that may limit its distribution
We acknowledge the Scottish Government Rural Environment Research and Analysis Directorate for support. Mireia Lopez-Siles was awarded a fellowship from the Spanish Ministerio de Educación to support a research stay at the RINH and is also the recipient of an FI grant from the Generalitat de Catalunya (2010FI_B2 00135), which receives support from the European Union Commissionate. This work was partially funded by the Spanish Ministry of Education and Science through project SAF2006-00414
Format: application/pdf
ISSN: 0099-2240 (versió paper)
1098-5336 (versió electrònica)
Document access: http://hdl.handle.net/10256/12242
Language: eng
Publisher: American Society for Microbiology
Collection: CICYT/PN 2006-2009/SAF2006-00414
Reproducció digital del document publicat a: http://dx.doi.org/10.1128/AEM.06858-11
Articles publicats (D-B)
Is part of: © Applied and Environmental Microbiology, 2012, vol. 78, p. 420-428
Rights: Tots els drets reservats
Subject: Còlon
Colon (Anatomy)
Còlon -- Microbiologia
Colon (Anatomy) -- Microbiology
Bacteris -- Creixement
Bacterial growth
Intestins -- Inflamació
Inflammatory bowel diseases
Title: Cultured representatives of two major phylogroups of human colonic Faecalibacterium prausnitzii can utilize pectin, uronic acids, and host-derived substrates for growth
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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