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The effects of sediment depth and oxygen concentration on the use of organic matter: An experimental study using an infiltration sediment tank

Water flowing through hyporheic river sediments or artificial recharge facilities promotes the development of microbial communities with sediment depth. We performed an 83-day mesocosm infiltration experiment, to study how microbial functions (e.g., extracellular enzyme activities and carbon substrate utilization) are affected by sediment depth (up to 50. cm) and different oxygen concentrations. Results indicated that surface sediment layers were mainly colonized by microorganisms capable of using a wide range of substrates (although they preferred to degrade carbon polymeric compounds, as indicated by the higher β-glucosidase activity). In contrast, at a depth of 50. cm, the microbial community became specialized in using fewer carbon substrates, showing decreased functional richness and diversity. At this depth, microorganisms picked nitrogenous compounds, including amino acids and carboxyl acids. After the 83-day experiment, the sediment at the bottom of the tank became anoxic, inhibiting phosphatase activity. Coexistence of aerobic and anaerobic communities, promoted by greater physicochemical heterogeneity, was also observed in deeper sediments. The presence of specific metabolic fingerprints under oxic and anoxic conditions indicated that the microbial community was adapted to use organic matter under different oxygen conditions. Overall the heterogeneity of oxygen concentrations with depth and in time would influence organic matter metabolism in the sediment tank

This work has been performed in the framework of project SCARCE Consolider-Ingenio 2010 program (reference CSD2009-00065). A. F. benefited from a grant (FI-DGR 2013) from the Catalan Government. X. S. acknowledges support from ICREA Academia. D. F-G acknowledges the financial support provided by the Spanish Ministry of Science through project FEAR (CGL2012-38120). AM R. acknowledges the financial support provided by the Spanish Ministry of Economy and Competitiveness through project FLUMED-HOTSPOTS (CGL2011-30151-C02). SR acknowledges the support of the Provincia Autonoma di Trento and the European Commission. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no PCOFUND-GA-2008-226070. We also thank anonymous reviewers for their suggestions

© Science of the Total Environment, 2015, vol. 540, p. 20-31

Elsevier

Manager: Ministerio de Ciencia e Innovación (Espanya)
Generalitat de Catalunya. Agència de Gestió d’Ajuts Universitaris i de Recerca
Author: Freixa Casals, Anna
Rubol, Simonetta
Carles Brangari, Albert
Fernandez Garcia, Daniel
Butturini, Andrea
Sánchez Vila, Xavier
Romaní i Cornet, Anna M.
Date: 2015 April 18
Abstract: Water flowing through hyporheic river sediments or artificial recharge facilities promotes the development of microbial communities with sediment depth. We performed an 83-day mesocosm infiltration experiment, to study how microbial functions (e.g., extracellular enzyme activities and carbon substrate utilization) are affected by sediment depth (up to 50. cm) and different oxygen concentrations. Results indicated that surface sediment layers were mainly colonized by microorganisms capable of using a wide range of substrates (although they preferred to degrade carbon polymeric compounds, as indicated by the higher β-glucosidase activity). In contrast, at a depth of 50. cm, the microbial community became specialized in using fewer carbon substrates, showing decreased functional richness and diversity. At this depth, microorganisms picked nitrogenous compounds, including amino acids and carboxyl acids. After the 83-day experiment, the sediment at the bottom of the tank became anoxic, inhibiting phosphatase activity. Coexistence of aerobic and anaerobic communities, promoted by greater physicochemical heterogeneity, was also observed in deeper sediments. The presence of specific metabolic fingerprints under oxic and anoxic conditions indicated that the microbial community was adapted to use organic matter under different oxygen conditions. Overall the heterogeneity of oxygen concentrations with depth and in time would influence organic matter metabolism in the sediment tank
This work has been performed in the framework of project SCARCE Consolider-Ingenio 2010 program (reference CSD2009-00065). A. F. benefited from a grant (FI-DGR 2013) from the Catalan Government. X. S. acknowledges support from ICREA Academia. D. F-G acknowledges the financial support provided by the Spanish Ministry of Science through project FEAR (CGL2012-38120). AM R. acknowledges the financial support provided by the Spanish Ministry of Economy and Competitiveness through project FLUMED-HOTSPOTS (CGL2011-30151-C02). SR acknowledges the support of the Provincia Autonoma di Trento and the European Commission. This project has received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no PCOFUND-GA-2008-226070. We also thank anonymous reviewers for their suggestions
Format: application/pdf
Citation: 023947
ISSN: 0048-9697
Document access: http://hdl.handle.net/10256/12262
Language: eng
Publisher: Elsevier
Collection: MICINN/PN 2009-2014/CSD2009-00065
AGAUR/2013 FI-DGR
MICINN/PN 2012-2014/CGL2011-30151-C02
Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.scitotenv.2015.04.007
Articles publicats (D-CCAA)
Is part of: © Science of the Total Environment, 2015, vol. 540, p. 20-31
Rights: Tots els drets reservats
Subject: Ecologia experimental
Experimental ecology
Microorganismes
Microorganisms
Ecologia microbiana
Microbial ecology
Title: The effects of sediment depth and oxygen concentration on the use of organic matter: An experimental study using an infiltration sediment tank
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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