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High CO2 concentration as an inductor agent to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories

Cationic α-helical antimicrobial peptides such as BP100 are of increasing interest for developing novel phytosanitary or therapeutic agents and products with industrial applications. Biotechnological production of these peptides in plants can be severely impaired due to the toxicity exerted on the host by high-level expression. This can be overcome by using inducible promoters with extremely low activity throughout plant development, although the yields are limited. We examined the use of modified atmospheres using the increased levels of [CO2], commonly used in the food industry, as the inductor agent to biotechnologically produce phytotoxic compounds with higher yields. Here we show that 30 % [CO2] triggered a profound transcriptional response in rice leaves, including a change in the energy provision from photosynthesis to glycolysis, and the activation of stress defense mechanisms. Five genes with central roles in up-regulated pathways were initially selected and their promoters successfully used to drive the expression of phytotoxic BP100 in genetically modified (GM) rice. GM plants had a normal phenotype on development and seed production in non-induction conditions. Treatment with 30 % [CO2] led to recombinant peptide accumulation of up to 1 % total soluble protein when the Os.hb2 promoter was used. This is within the range of biotechnological production of other peptides in plants. Using BP100 as a proof-of-concept we demonstrate that very high [CO2] can be considered an economically viable strategy to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories

This work was supported by the Spanish MCI AGL2010-17181/AGR and MEC AGL2013-41405-R projects

© Plant Molecular Biology, 2016, vol. 90, núm.4-5, p. 329-343

Springer Verlag

Author: Ruiz Ramírez, Cristina
Pla i de Solà-Morales, Maria
Company Casadevall, Núria
Riudavets, Jordi
Nadal i Matamala, Anna
Date: 2016
Abstract: Cationic α-helical antimicrobial peptides such as BP100 are of increasing interest for developing novel phytosanitary or therapeutic agents and products with industrial applications. Biotechnological production of these peptides in plants can be severely impaired due to the toxicity exerted on the host by high-level expression. This can be overcome by using inducible promoters with extremely low activity throughout plant development, although the yields are limited. We examined the use of modified atmospheres using the increased levels of [CO2], commonly used in the food industry, as the inductor agent to biotechnologically produce phytotoxic compounds with higher yields. Here we show that 30 % [CO2] triggered a profound transcriptional response in rice leaves, including a change in the energy provision from photosynthesis to glycolysis, and the activation of stress defense mechanisms. Five genes with central roles in up-regulated pathways were initially selected and their promoters successfully used to drive the expression of phytotoxic BP100 in genetically modified (GM) rice. GM plants had a normal phenotype on development and seed production in non-induction conditions. Treatment with 30 % [CO2] led to recombinant peptide accumulation of up to 1 % total soluble protein when the Os.hb2 promoter was used. This is within the range of biotechnological production of other peptides in plants. Using BP100 as a proof-of-concept we demonstrate that very high [CO2] can be considered an economically viable strategy to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories
This work was supported by the Spanish MCI AGL2010-17181/AGR and MEC AGL2013-41405-R projects
Format: application/pdf
ISSN: 0167-4412 (versió paper)
1573-5028 (versió electrònica)
Document access: http://hdl.handle.net/10256/12339
Language: eng
Publisher: Springer Verlag
Collection: MICINN/PN 2011-2013/AGL2010-17181
MINECO/PE 2014-2016/AGL2013-41405-R
Reproducció digital del document publicat a: http://dx.doi.org/10.1007/s11103-015-0419-6
Articles publicats (D-EQATA)
Is part of: © Plant Molecular Biology, 2016, vol. 90, núm.4-5, p. 329-343
Rights: Tots els drets reservats
Subject: Plantes transgèniques
Transgenic plants
Antibiòtics pèptids
Peptide antibiotics
Title: High CO2 concentration as an inductor agent to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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