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Ministerio de Ciencia e Innovación (Espanya)
Ministerio de EconomÃa y Competitividad (Espanya) |
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Kurpiewska, Katarzyna
Dziubek, Kamil Katrusiak, Andrzej Font i SadurnÃ, Josep Ribó i Panosa, Marc Vilanova i Brugués, Maria LewiÅ„ski, Krzysztof |
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2016 | |
Hydrostatic pressure in range 0.1-1.5 GPa is used to modify biological system behaviour mostly in biophysical studies of proteins in solution. Due to specific influence on the system equilibrium high pressure can act as a filter that enables to identify and investigate higher energy protein conformers. The idea of the presented experiments is to examine the behaviour of RNase A molecule under high pressure before and after introduction of destabilizing mutation. For the first time crystal structures of wild-type bovine pancreatic ribonuclease A and its markedly less stable variant modified at position Ile106 were determined at different pressures. X-ray diffraction experiments at high pressure showed that the secondary structure of RNase A is well preserved even beyond 0.67 GPa at room temperature. Detailed structural analysis of ribonuclease A conformation observed under high pressure revealed that pressure influences hydrogen bonds pattern, cavity size and packing of molecule This work has been supported by Grants BFU2009-06935 and BIO2013-43517 from MINECO (Spain) |
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application/pdf | |
http://hdl.handle.net/10256/12346 | |
eng | |
Elsevier | |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphys.2016.01.010 info:eu-repo/semantics/altIdentifier/issn/0301-0104 info:eu-repo/grantAgreement/MICINN//BFU2009-06935/ES/Bases Moleculares Del Plegamiento Y Citotoxicidad De Las Ribonucleasas Pancreaticas. Evaluacion De La Actividad Citotoxica Y Diseño De Estrategias Para Su Control Mediante Splicing Proteico./ info:eu-repo/grantAgreement/MINECO//BIO2013-43517-R/ES/RIBONUCLEASAS E INTEINAS COMO HERRAMIENTAS MOLECULARES PARA EL DESARROLLO DE FARMACOS ANTITUMORALES Y ESTUDIO DE PROTEINOPATIAS/ |
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Tots els drets reservats | |
Ribonuclease A
Ribonucleases Enzims Enzymes Enginyeria de proteïnes Protein engineering |
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Structural investigation of ribonuclease A conformational preferences using high pressure protein crystallography | |
info:eu-repo/semantics/article | |
DUGiDocs |