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Seasonal variation in transcript abundance in cork tissue analyzed by real time RT-PCR

The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate genes for cork biosynthesis and regulationwas made available opening newpossibilities for molecular studies in cork oak (Quercus suber L.). Based on this list, we analyzed the seasonal variation inmRNAabundance in cork tissue of selected genes by real time reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript abundance was evaluated by principal component analysis and genes were clustered in several functional subgroups. Structural genes of suberin pathways such as CYP86A1, GPAT and HCBT, and regulatory genes of the NAMand WRKY families showed highest transcript accumulation in June, a crucial month for cork development. Other cork structural genes, such as FAT and F5H, were significantly correlated with temperature and relative humidity. The stress genes HSP17.4 and ANNwere strongly positively correlated to temperature, in accord with their protective role

The work was supported by funding from Spanish Ministerio de Ciencia y Tecnología (AGL2003–00416) and from the Ministerio de Educación y Ciencia (AGL2006-07342), the European Social Funds and the Departament d’Universitats, Investigació i Societat de la Informació of Catalonia

© Tree Physiology, 2008, vol. 28, núm. 5, p. 743-751

Oxford University Press (OUP)

Author: Soler, Marçal
Serra i Figueras, Olga
Molinas de Ferrer, Maria Lluïsa
García-Berthou, Emili
Caritat, Antònia
Figueras i Vall-llosera, Mercè
Date: 2008
Abstract: The molecular processes underlying cork biosynthesis and differentiation are mostly unknown. Recently, a list of candidate genes for cork biosynthesis and regulationwas made available opening newpossibilities for molecular studies in cork oak (Quercus suber L.). Based on this list, we analyzed the seasonal variation inmRNAabundance in cork tissue of selected genes by real time reverse-transcriptase polymerase chain reaction (RT-PCR). Relative transcript abundance was evaluated by principal component analysis and genes were clustered in several functional subgroups. Structural genes of suberin pathways such as CYP86A1, GPAT and HCBT, and regulatory genes of the NAMand WRKY families showed highest transcript accumulation in June, a crucial month for cork development. Other cork structural genes, such as FAT and F5H, were significantly correlated with temperature and relative humidity. The stress genes HSP17.4 and ANNwere strongly positively correlated to temperature, in accord with their protective role
The work was supported by funding from Spanish Ministerio de Ciencia y Tecnología (AGL2003–00416) and from the Ministerio de Educación y Ciencia (AGL2006-07342), the European Social Funds and the Departament d’Universitats, Investigació i Societat de la Informació of Catalonia
Format: application/pdf
ISSN: 0829-318X (versió paper)
1758-4469 (versió electrònica)
Document access: http://hdl.handle.net/10256/12383
Language: eng
Publisher: Oxford University Press (OUP)
Collection: MICYT/2003-2006/AGL2003-00416
MEC/PN 2006-2009/ AGL2006-07342/AGR
Reproducció digital del document publicat a: http://dx.doi.org/10.1093/treephys/28.5.743
Articles publicats (D-B)
Is part of: © Tree Physiology, 2008, vol. 28, núm. 5, p. 743-751
Rights: Tots els drets reservats
Subject: Suro
Cork
Biosíntesi
Biosynthesis
Title: Seasonal variation in transcript abundance in cork tissue analyzed by real time RT-PCR
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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