Item


A PCR technique to detect enterotoxigenic and verotoxigenic Escherichia coli in boar semen samples

In semen, bacteria’s isolation from a pure culture is complex, laborious and easily alterable by the presence of antibiotics and inhibitors. We developed a PCR technique to detect the presence of the enterotoxigenic (ETEC) and verotoxigenic Escherichia coli (VTEC) (strains with high prevalence in the swine industry) in semen by adapting the protocols developed by Zhang et al. (2007) and Yilmaz et al. (2006). We artificially inoculated extended semen samples at different infective concentrations of bacteria (from 10 2 to 10 8 bacteriaml -1) with two enterotoxigenic and verotoxigenic strains, and performed two multiplex and one conventional PCR. This technique proved to be a quick, useful and reliable tool to detect the presence of ETEC and VTEC up to an infective dose of 10 5 bacteriaml -1 in semen

This research was supported by Project RZ2008-0001-00-00, funds from the ‘‘Ministerio de Ciencia e Innovación’’ (Spanish Government)

© Research in Veterinary Science, 2012, vol. 93, núm. 1, p. 31-33

Elsevier

Author: Bussalleu Muntada, Eva
Pinart Nadal, Elisabeth
Yeste Oliveras, Marc
Briz González, Maria Dolors
Sancho Badell, Sílvia
Torner Bosch, Eva
Bonet, Sergi
Date: 2012
Abstract: In semen, bacteria’s isolation from a pure culture is complex, laborious and easily alterable by the presence of antibiotics and inhibitors. We developed a PCR technique to detect the presence of the enterotoxigenic (ETEC) and verotoxigenic Escherichia coli (VTEC) (strains with high prevalence in the swine industry) in semen by adapting the protocols developed by Zhang et al. (2007) and Yilmaz et al. (2006). We artificially inoculated extended semen samples at different infective concentrations of bacteria (from 10 2 to 10 8 bacteriaml -1) with two enterotoxigenic and verotoxigenic strains, and performed two multiplex and one conventional PCR. This technique proved to be a quick, useful and reliable tool to detect the presence of ETEC and VTEC up to an infective dose of 10 5 bacteriaml -1 in semen
This research was supported by Project RZ2008-0001-00-00, funds from the ‘‘Ministerio de Ciencia e Innovación’’ (Spanish Government)
Format: application/pdf
ISSN: 0034-5288 (versió paper)
1532-2661 (versió electrònica)
Document access: http://hdl.handle.net/10256/12394
Language: eng
Publisher: Elsevier
Collection: MICINN/PN 2011-2014/RZ 2011-00001-00-00
Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.rvsc.2011.07.012
Articles publicats (D-B)
Is part of: © Research in Veterinary Science, 2012, vol. 93, núm. 1, p. 31-33
Rights: Tots els drets reservats
Subject: Reacció en cadena de la polimerasa
Polymerase chain reaction
Porcs -- Espermatozoides
Swine -- Spermatozoa
Infeccions per escheríchia coli
Escherichia coli infections
Title: A PCR technique to detect enterotoxigenic and verotoxigenic Escherichia coli in boar semen samples
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

Subjects

Authors