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Modeling gene flow distribution within conventional fields and development of a simplified sampling method to quantify adventitious GM contents in maize

Cationic a-helical antimicrobial peptides such as BP100 are of increasing interest for developing novel phytosanitary or therapeutic agents and products with industrial applications. Biotechnological production of these peptides in plants can be severely impaired due to the toxicity exerted on the host by high-level expression. This can be overcome by using inducible promoters with extremely low activity throughout plant development, although the yields are limited. We examined the use of modified atmospheres using the increased levels of [CO2], commonly used in the food industry, as the inductor agent to biotechnologically produce phytotoxic compounds with higher yields. Here we show that 30 % [CO2] triggered a profound transcriptional response in rice leaves, including a change in the energy provision from photosynthesis to glycolysis, and the activation of stress defense mechanisms. Five genes with central roles in up-regulated pathways were initially selected and their promoters successfully used to drive the expression of phytotoxic BP100 in genetically modified (GM) rice. GM plants had a normal phenotype on development and seed production in non-induction conditions. Treatment with 30 % [CO2] led to recombinant peptide accumulation of up to 1 % total soluble protein when the Os.hb2 promoter was used. This is within the range of biotechnological production of other peptides in plants. Using BP100 as a proof-of-concept we demonstrate that very high [CO2] can be considered an economically viable strategy to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories

Scientific Reports, 2015, vol. 5, núm. art. 17106

Nature Publishing Group

Author: Melé, Enric
Nadal i Matamala, Anna
Messeguer i Peypoch, Joaquima
Melé-Messeguer, Marina
Palaudelmàs, Montserrat
Peñas, Gisela
Piferrer, Xavier
Capellades, Gemma
Serra, Joan
Pla i de Solà-Morales, Maria
Date: 2015
Abstract: Cationic a-helical antimicrobial peptides such as BP100 are of increasing interest for developing novel phytosanitary or therapeutic agents and products with industrial applications. Biotechnological production of these peptides in plants can be severely impaired due to the toxicity exerted on the host by high-level expression. This can be overcome by using inducible promoters with extremely low activity throughout plant development, although the yields are limited. We examined the use of modified atmospheres using the increased levels of [CO2], commonly used in the food industry, as the inductor agent to biotechnologically produce phytotoxic compounds with higher yields. Here we show that 30 % [CO2] triggered a profound transcriptional response in rice leaves, including a change in the energy provision from photosynthesis to glycolysis, and the activation of stress defense mechanisms. Five genes with central roles in up-regulated pathways were initially selected and their promoters successfully used to drive the expression of phytotoxic BP100 in genetically modified (GM) rice. GM plants had a normal phenotype on development and seed production in non-induction conditions. Treatment with 30 % [CO2] led to recombinant peptide accumulation of up to 1 % total soluble protein when the Os.hb2 promoter was used. This is within the range of biotechnological production of other peptides in plants. Using BP100 as a proof-of-concept we demonstrate that very high [CO2] can be considered an economically viable strategy to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories
Format: application/pdf
ISSN: 2045-2322
Document access: http://hdl.handle.net/10256/12486
Language: eng
Publisher: Nature Publishing Group
Collection: Reproducció digital del document publicat a: http://dx.doi.org/10.1038/srep17106
Articles publicats (D-EQATA)
Is part of: Scientific Reports, 2015, vol. 5, núm. art. 17106
Rights: Reconeixement 3.0 Espanya
Rights URI: http://creativecommons.org/licenses/by/3.0/es
Subject: Plantes transgèniques
Transgenic plants
Blat de moro -- Genètica
Corn -- Genetics
Title: Modeling gene flow distribution within conventional fields and development of a simplified sampling method to quantify adventitious GM contents in maize
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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