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Development of an extraction and purification method for the determination of multi-class pharmaceuticals and endocrine disruptors in freshwater invertebrates

Aquatic organisms from freshwater ecosystems impacted by waste water treatment plant (WWTP) effluents are constantly exposed to constant concentrations of pharmaceuticals, endocrine disruptors and related compounds, among other anthropogenic contaminants. Macroinvertebrates inhabiting freshwater ecosystems might be useful bioindicators of exposure to contaminants, since their lives are long enough to bioaccumulate, but at the same time may integrate short-term changes in the environment. However, studies about potential bioaccumulation of emerging contaminants in these organisms are very scarce. The objectives of this study were to develop an analytical methodology for the analysis of 41 pharmaceuticals and 21 endocrine disruptors in freshwater invertebrates. In addition, bioaccumulation of these contaminants in three macroinvertebrate taxa inhabiting a waste water treatment plant -impacted river was evaluated. The method for the simultaneous extraction of both families of compounds is based on sonication, purification via removal of phospholipids, and analysis by ultra performance liquid chromatography coupled to a mass spectrometer (UPLC-MS/MS) in tandem. Recoveries for pharmaceuticals were 34-125%, and for endocrine disruptors were 48-117%. Method detection limits (MDLs) for EDCs were in the range of 0.080-2.4 ng g-1, and for pharmaceuticals, 0.060-4.3 ng g-1. These pollutants were detected in water samples taken downstream the waste water treatment plant effluent at concentrations up to 572 ng L-1. Two non-esteroidal anti-inflammatory drugs, diclofenac and ibuprofen, and four endocrine disruptors - estrone, bisphenol A, TBEP, and nonylphenol - were detected in at least one macroinvertebrate taxa in concentrations up to 183 ng g-1 (dry weight). An isobaric interference was identified during the analysis of diclofenac in Hydropsyche samples, which was successfully discriminated via accurate mass determination by TFC-LTQ Orbitrap

This study has been financed by Spanish Ministry o fEconomy and Competitiveness through the project SCARCE (Consolider-Ingenio 2010CSD200900065). This work was partly supported by the Generalitat de Catalunya (Consolidated Research Group: Catalan Institute for water Research 2014 SGR 291)

© Talanta, 2015, vol. 132, p. 373-381

Elsevier

Author: Huerta Buitrago, Belinda
Jakimska, Anna
Llorca, Marta
Ruhí i Vidal, Albert
Margoutidis, George
Acuña i Salazar, Vicenç
Sabater, Sergi
Rodríguez Mozaz, Sara
Barceló i Cullerés, Damià
Date: 2015 January 15
Abstract: Aquatic organisms from freshwater ecosystems impacted by waste water treatment plant (WWTP) effluents are constantly exposed to constant concentrations of pharmaceuticals, endocrine disruptors and related compounds, among other anthropogenic contaminants. Macroinvertebrates inhabiting freshwater ecosystems might be useful bioindicators of exposure to contaminants, since their lives are long enough to bioaccumulate, but at the same time may integrate short-term changes in the environment. However, studies about potential bioaccumulation of emerging contaminants in these organisms are very scarce. The objectives of this study were to develop an analytical methodology for the analysis of 41 pharmaceuticals and 21 endocrine disruptors in freshwater invertebrates. In addition, bioaccumulation of these contaminants in three macroinvertebrate taxa inhabiting a waste water treatment plant -impacted river was evaluated. The method for the simultaneous extraction of both families of compounds is based on sonication, purification via removal of phospholipids, and analysis by ultra performance liquid chromatography coupled to a mass spectrometer (UPLC-MS/MS) in tandem. Recoveries for pharmaceuticals were 34-125%, and for endocrine disruptors were 48-117%. Method detection limits (MDLs) for EDCs were in the range of 0.080-2.4 ng g-1, and for pharmaceuticals, 0.060-4.3 ng g-1. These pollutants were detected in water samples taken downstream the waste water treatment plant effluent at concentrations up to 572 ng L-1. Two non-esteroidal anti-inflammatory drugs, diclofenac and ibuprofen, and four endocrine disruptors - estrone, bisphenol A, TBEP, and nonylphenol - were detected in at least one macroinvertebrate taxa in concentrations up to 183 ng g-1 (dry weight). An isobaric interference was identified during the analysis of diclofenac in Hydropsyche samples, which was successfully discriminated via accurate mass determination by TFC-LTQ Orbitrap
This study has been financed by Spanish Ministry o fEconomy and Competitiveness through the project SCARCE (Consolider-Ingenio 2010CSD200900065). This work was partly supported by the Generalitat de Catalunya (Consolidated Research Group: Catalan Institute for water Research 2014 SGR 291)
Format: application/pdf
ISSN: 0039-9140
Document access: http://hdl.handle.net/10256/12579
Language: eng
Publisher: Elsevier
Collection: MICINN/PN 2009-2014/CSD2009-00065
Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.talanta.2014.09.017
Articles publicats (D-CCAA)
Is part of: © Talanta, 2015, vol. 132, p. 373-381
Rights: Tots els drets reservats
Subject: Disruptors endocrins
Endocrine disrupting chemicals
Invertebrats d’aigua dolça -- Efecte dels medicaments
Freshwater invertebrates -- Effect of drugs on
Invertebrats d’aigua dolça -- Efecte dels productes químics
Freshwater invertebrates -- Effect of chemicals on
Invertebrats d’aigua dolça -- Efecte de la contaminació de l’aigua
Freshwater invertebrates -- Effect of water pollution on
Contaminants emergents en l’aigua
Emerging contaminants in water
Title: Development of an extraction and purification method for the determination of multi-class pharmaceuticals and endocrine disruptors in freshwater invertebrates
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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