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Laccase-based biosensors for detection of phenolic compounds

Monitoring of phenolic compounds in the food industry and for environmental and medical applications has become more relevant in recent years. Conventional methods for detection and quantification of these compounds, such as spectrophotometry and chromatography, are time consuming and expensive. However, laccase biosensors represent a fast method for on-line and in situ monitoring of these compounds. We discuss the main transduction principles. We divide the electrochemical principle into amperometric, voltammetric, potentiometric and conductometric sensors. We divide optical transducers into fluorescence and absorption. The amperometric transducer method is the most widely studied and used for laccase biosensors. Optical biosensors present higher sensitivity than the other biosensors. Laccase production is dominated by a few fungus genera: Trametes, Aspergillus, and Ganoderma. We present an overview of laccase biosensors used for the determination of phenolic compounds in industrial applications

TrAC : trends in analytical chemistry, 2015, vol. 74, p. 21-45

Elsevier

Author: Rodríguez Delgado, Melissa M.
Alemán Nava, Gibrán S.
Rodríguez Delgado, José Manuel
Dieck-Assad, Graciano
Martínez Chapa, Sergio Omar
Barceló i Cullerés, Damià
Parra, Roberto
Date: 2015 December
Abstract: Monitoring of phenolic compounds in the food industry and for environmental and medical applications has become more relevant in recent years. Conventional methods for detection and quantification of these compounds, such as spectrophotometry and chromatography, are time consuming and expensive. However, laccase biosensors represent a fast method for on-line and in situ monitoring of these compounds. We discuss the main transduction principles. We divide the electrochemical principle into amperometric, voltammetric, potentiometric and conductometric sensors. We divide optical transducers into fluorescence and absorption. The amperometric transducer method is the most widely studied and used for laccase biosensors. Optical biosensors present higher sensitivity than the other biosensors. Laccase production is dominated by a few fungus genera: Trametes, Aspergillus, and Ganoderma. We present an overview of laccase biosensors used for the determination of phenolic compounds in industrial applications
Format: application/pdf
ISSN: 0165-9936
Document access: http://hdl.handle.net/10256/12596
Language: eng
Publisher: Elsevier
Collection: Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.trac.2015.05.008
Articles publicats (ICRA)
Is part of: TrAC : trends in analytical chemistry, 2015, vol. 74, p. 21-45
Rights: Attribution 3.0 Spain
Rights URI: http://creativecommons.org/licenses/by/3.0/es/
Subject: Biosensors
Biosensors
Absorció
Absorption
Fenols
Phenols
Aliments -- Anàlisi
Food -- Analysis
Title: Laccase-based biosensors for detection of phenolic compounds
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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