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Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus.

The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource

PeerJ, 2016, vol. e2200, p. 1-14

Wiley

Manager: Ministerio de Ciencia e Innovación (Espanya)
Ministerio de Economía y Competitividad (Espanya)
Author: Heras Mena, Sandra
Planella Albi, Laia
Caldarazzo, Ilaria
Vera Rodríguez, Manuel
García Marín, José Luis
Roldán Borassi, María Inés
Date: 2016 July 27
Abstract: The blue and red shrimp, Aristeus antennatus, is a commercially important crustacean, in the Mediterranean Sea, which has been listed as a priority species for fishery management. Hypervariable microsatellite markers could be a useful tool to identify genetic stocks among geographically close fishing grounds. Potential microsatellite markers (97) identified from next-generation sequencing of an individual shrimp using a 454 GS Junior Pyrosequencer were tested on a preliminary panel of 15 individuals representing the four worldwide genetic stocks of the species from which 35 polymorphic loci were identified and used to characterize an additional 20 individuals from the Western Mediterranean Sea. In the Western Mediterranean sample, 32 out of 35 were polymorphic loci and the number of alleles per locus ranged from 2 to 14 and expected heterozygosity ranged from 0.050 to 0.968. No linkage disequilibrium was detected, indicating the independence of the loci. These novel microsatellites provide additional tools to address questions relating to genetic diversity, parentage studies and connectivity patterns of A. antennatus populations and help develop effective strategies to ensure long-term sustainability of this resource
Format: application/pdf
Citation: 025448
ISSN: 2167-8359
Document access: http://hdl.handle.net/10256/12633
Language: eng
Publisher: Wiley
Collection: MICINN/PN 2010-2013/AGL2009-09228
MINECO/PE 2015-2017/CTM2014-54648-C2-2-R
Reproducció digital del document publicat a: http://dx.doi.org/10.7717/peerj.2200
Articles publicats (D-B)
Is part of: PeerJ, 2016, vol. e2200, p. 1-14
Rights: Reconeixement 4.0 Internacional
Rights URI: http://creativecommons.org/licenses/by/4.0
Subject: Biologia de la conservació
Conservation biology
Biologia marina
Marine biology
Genètica de poblacions
Population genetics
Gambes -- Genètica -- Mediterrània, Mar
Shrimps -- Genetics -- Mediterranean Sea
Title: Development and characterization of novel microsatellites markers by next generation sequencing for the blue and red shrimp Aristeus antennatus.
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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