Ítem


Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds

The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60° scaffolds were more suitable for 3D culture than the 45° and 90° ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs population

This work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036)

Molecules, 2016, vol. 21, núm. 4, p. 537

MDPI (Multidisciplinary Digital Publishing Institute)

Autor: Palomeras, Sònia
Rabionet Díaz, Marc
Ferrer Real, Inés
Sarrats Carbó, Ariadna
Garcia-Romeu, Maria Luisa
Puig i Miquel, Teresa
Ciurana, Quim de
Data: 2016
Resum: The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs’ phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60° scaffolds were more suitable for 3D culture than the 45° and 90° ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs population
This work was supported partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and Ministerio de Economía Y Competitividad (DPI2013-45201-P), and the support of the Catalonian government (2014SGR00868). The authors are grateful for the financial support from the University of Girona (MPCUdG2016/036)
Format: application/pdf
Cita: 025307
ISSN: 1420-3049
Accés al document: http://hdl.handle.net/10256/12961
Llenguatge: eng
Editor: MDPI (Multidisciplinary Digital Publishing Institute)
Col·lecció: MINECO/PE 2014-2016/DPI2013-45201-P
Reproducció digital del document publicat a: http://dx.doi.org/10.3390/molecules21040537
Articles publicats (D-CM)
És part de: Molecules, 2016, vol. 21, núm. 4, p. 537
Drets: Attribution 4.0 Spain
URI Drets: http://creativecommons.org/licenses/by/4.0/es/
Matèria: Mama -- Càncer
Breast -- Cancer
Cèl·lules mare -- Càncer
Stem cells -- Cancer
Títol: Breast Cancer Stem Cell Culture and Enrichment Using Poly("-Caprolactone) Scaffolds
Tipus: info:eu-repo/semantics/article
Repositori: DUGiDocs

Matèries

Autors