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Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria

The antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin), fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide products have been studied in a collection of 64 strains of Bacillus spp. isolated from plant environments. The most frequent antimicrobial peptide (AMP) genes were bmyB, srfAAand fenD (34-50% of isolates). Most isolates (98.4%) produced surfactin isoforms, 90.6%iturins and 79.7% fengycins. The antibacterial activity was very frequent and generally intense among the collection of strains because 75% of the isolates were active against at least 6 of the 8 bacterial plant pathogens tested. Hierarchical and correspondence analysis confirmed the presence of two clearly differentiated groups. One group consisted of Bacillus strains that showed a strong antibacterial activity, presented several cLPs genes and produced several isoforms of cLPs simultaneously, mainly composed of B. subtilisand B. amyloliquefaciens, although the last one was exclusive to this group. Another group was characterized by strains with very low or none antibacterial activity, that showed one or none of the cLP genes and produced a few or none of the corresponding cLPs, and was the most heterogenous group including B. subtilis, B. licheniformis, B. megaterium, B. pumilus, B. cereusand B. thuringiensis, although the last two were exclusive to this group. This work demonstrated that the antagonistic capacity of plant-associated Bacillusagainst plant pathogenic bacteria is related to the presence of cLP genes and to the production of the corresponding cLPs, and it is mainly associated to the species B. subtilisand B. amyloliquefaciens. Our findings would help to increase the yield and efficiency of screening methods to obtain candidate strains to biocontrol agents with a mechanism of action relaying on the production of antimicrobial cLPs

Funding was provided by Programa Nacional de Agricultura y Alimentación (CICYT) (Ref. AGL-2009-13255-C02-01) of the MINECO of Spain, and FP7 KBBE.2013.1.2-04. Ref. 613678. DROPSA project of the European Union. The research group is accredited and supported in part by SGR 2009-0812, 2014 SGR 697 and TECNIO net from Catalonia

PLoS One, 2015, vol. 10, núm. 5, p. e0127738

Public Library of Science (PLoS)

Autor: Mora Pons, Isabel
Cabrefiga Olamendi, Jordi
Montesinos Seguí, Emilio
Data: 2015
Resum: The antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin), fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide products have been studied in a collection of 64 strains of Bacillus spp. isolated from plant environments. The most frequent antimicrobial peptide (AMP) genes were bmyB, srfAAand fenD (34-50% of isolates). Most isolates (98.4%) produced surfactin isoforms, 90.6%iturins and 79.7% fengycins. The antibacterial activity was very frequent and generally intense among the collection of strains because 75% of the isolates were active against at least 6 of the 8 bacterial plant pathogens tested. Hierarchical and correspondence analysis confirmed the presence of two clearly differentiated groups. One group consisted of Bacillus strains that showed a strong antibacterial activity, presented several cLPs genes and produced several isoforms of cLPs simultaneously, mainly composed of B. subtilisand B. amyloliquefaciens, although the last one was exclusive to this group. Another group was characterized by strains with very low or none antibacterial activity, that showed one or none of the cLP genes and produced a few or none of the corresponding cLPs, and was the most heterogenous group including B. subtilis, B. licheniformis, B. megaterium, B. pumilus, B. cereusand B. thuringiensis, although the last two were exclusive to this group. This work demonstrated that the antagonistic capacity of plant-associated Bacillusagainst plant pathogenic bacteria is related to the presence of cLP genes and to the production of the corresponding cLPs, and it is mainly associated to the species B. subtilisand B. amyloliquefaciens. Our findings would help to increase the yield and efficiency of screening methods to obtain candidate strains to biocontrol agents with a mechanism of action relaying on the production of antimicrobial cLPs
Funding was provided by Programa Nacional de Agricultura y Alimentación (CICYT) (Ref. AGL-2009-13255-C02-01) of the MINECO of Spain, and FP7 KBBE.2013.1.2-04. Ref. 613678. DROPSA project of the European Union. The research group is accredited and supported in part by SGR 2009-0812, 2014 SGR 697 and TECNIO net from Catalonia
Format: application/pdf
ISSN: 1932-6203
Accés al document: http://hdl.handle.net/10256/13307
Llenguatge: eng
Editor: Public Library of Science (PLoS)
Col·lecció: MICINN/PN 2010-2012/ AGL2009-13255-C02-01
Reproducció digital del document publicat a: http://dx.doi.org/10.1371/journal.pone.0127738
Articles publicats (D-EQATA)
info:eu-repo/grantAgreement/EC/FP7/613678
És part de: PLoS One, 2015, vol. 10, núm. 5, p. e0127738
Drets: Attribution 4.0 Spain
URI Drets: http://creativecommons.org/licenses/by/4.0/es/
Matèria: Antibiòtics pèptids
Peptide antibiotics
Bacils
Bacillus (Bacteria)
Plantes -- Malalties
Plant diseases
Bacteris fitopatògens
Phytopathogenic bacteria
Títol: Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
Tipus: info:eu-repo/semantics/article
Repositori: DUGiDocs

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