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The Identification and Quantification of Suberin Monomers of Root and Tuber Periderm from Potato (Solanum tuberosum) as Fatty Acyl tert-Butyldimethylsilyl Derivatives

Protective plant lipophilic barriers such as suberin and cutin, with their associated waxes, are complex fatty acyl derived polyesters. Their precise chemical composition is valuable to understand the specific role of each compound to the physiological function of the barrier. Objectives: To develop a method for the compositional analysis of suberin and associated waxes by gas chromatography (GC) coupled to ion trap-mass spectrometry (IT-MS) using N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) as sylilating reagent, and apply it to compare the suberin of the root and tuber periderm of potato (Solanum tuberosum). Methodology: Waxes and suberin monomers from root and periderm were extracted subsequently using organic solvents and by methanolysis, and subjected to MTBSTFA derivatisation. GC analyses of periderm extracts were used to optimise the chromatographic method and the compound identification. Quantitative data was obtained using external calibration curves. The method was fully validated and applied for suberin composition analyses of roots and periderm. Results: Wax and suberin compounds were successfully separated and compound identification was based on the specific (M-57) and non-specific ions in mass spectra. The use of calibration curves built with different external standards provided quantitative accurate data and showed that suberin from root contains shorter chained fatty acyl derivatives and a relative predominance of α,ω-alkanedioic acids compared to that of the periderm. Conclusion: We present a method for the analysis of suberin and their associated waxes based on MTBSTFA derivatisation. Moreover, the characteristic root suberin composition may be the adaptive response to its specific regulation of permeability to water and gases

This work was financially supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN) (AGL2009-13745; CTM2011-28765-C02-02) and the Ministerio de Economía y Competitividad (MINECO) (AGL2012-36725), and the University of Girona (Accions Singulars 2011 (ASING2011)). DC-A also acknowledges a fellowship from the Patronat Francesc Eiximenis of the Diputació de Girona and a predoctoral grant from the University of Girona (BR10/27)

Wiley

Manager: Ministerio de Economía y Competitividad (Espanya)
Ministerio de Ciencia e Innovación (Espanya)
Author: Company Arumí, Dolors
Figueras i Vall-llosera, Mercè
Salvadó Martín, Victòria
Molinas de Ferrer, Maria Lluïsa
Serra i Figueras, Olga
Anticó i Daró, Ma. Enriqueta
Date: 2016 November
Abstract: Protective plant lipophilic barriers such as suberin and cutin, with their associated waxes, are complex fatty acyl derived polyesters. Their precise chemical composition is valuable to understand the specific role of each compound to the physiological function of the barrier. Objectives: To develop a method for the compositional analysis of suberin and associated waxes by gas chromatography (GC) coupled to ion trap-mass spectrometry (IT-MS) using N-(tert-butyldimethylsilyl)-N-methyl-trifluoroacetamide (MTBSTFA) as sylilating reagent, and apply it to compare the suberin of the root and tuber periderm of potato (Solanum tuberosum). Methodology: Waxes and suberin monomers from root and periderm were extracted subsequently using organic solvents and by methanolysis, and subjected to MTBSTFA derivatisation. GC analyses of periderm extracts were used to optimise the chromatographic method and the compound identification. Quantitative data was obtained using external calibration curves. The method was fully validated and applied for suberin composition analyses of roots and periderm. Results: Wax and suberin compounds were successfully separated and compound identification was based on the specific (M-57) and non-specific ions in mass spectra. The use of calibration curves built with different external standards provided quantitative accurate data and showed that suberin from root contains shorter chained fatty acyl derivatives and a relative predominance of α,ω-alkanedioic acids compared to that of the periderm. Conclusion: We present a method for the analysis of suberin and their associated waxes based on MTBSTFA derivatisation. Moreover, the characteristic root suberin composition may be the adaptive response to its specific regulation of permeability to water and gases
This work was financially supported by grants from the Spanish Ministerio de Ciencia e Innovación (MICINN) (AGL2009-13745; CTM2011-28765-C02-02) and the Ministerio de Economía y Competitividad (MINECO) (AGL2012-36725), and the University of Girona (Accions Singulars 2011 (ASING2011)). DC-A also acknowledges a fellowship from the Patronat Francesc Eiximenis of the Diputació de Girona and a predoctoral grant from the University of Girona (BR10/27)
Format: application/pdf
Document access: http://hdl.handle.net/10256/13403
Language: eng
Publisher: Wiley
Collection: info:eu-repo/semantics/altIdentifier/doi/10.1002/pca.2625
info:eu-repo/semantics/altIdentifier/issn/0958-0344
info:eu-repo/semantics/altIdentifier/eissn/1099-1565
info:eu-repo/grantAgreement/MINECO//AGL2012-36725/ES/NUEVAS ESTRATEGIAS TRANSCRIPTOMICAS PARA LOS GENES IMPLICADOS EN LA FORMACION DEL FELEMA/
info:eu-repo/grantAgreement/MICINN//CTM2011-28765-C02-02/ES/COMPORTAMIENTO Y ELIMINACION DE CONTAMINANTES ORGANICOS EMERGENTES EN SISTEMAS DE TRATAMIENTOS DE AGUAS. EVALUACION DE SISTEMAS TERCIARIOS./
Rights: Tots els drets reservats
Subject: Biopolímers -- Anàlisi
Biopolymers -- Analysis
Cromatografia de gasos
Gas chromatography
Biosíntesi
Biosynthesis
Title: The Identification and Quantification of Suberin Monomers of Root and Tuber Periderm from Potato (Solanum tuberosum) as Fatty Acyl tert-Butyldimethylsilyl Derivatives
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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