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Diversity of Miscellaneous Crenarchaeotic Group archaea in freshwater karstic lakes and their segregation between planktonic and sediment habitats

The Miscellaneous Crenarchaeotic Group (MCG) is an archaeal lineage whose members are widespread and abundant in marine sediments. MCG archaea have also been consistently found in stratified euxinic lakes. In this work, we have studied archaeal communities in three karstic lakes to reveal potential habitat segregation of MCG subgroups between planktonic and sediment compartments. In the studied lakes, archaeal assemblages were strikingly similar to those of the marine subsurface with predominance of uncultured Halobacteria in the plankton and Thermoplasmata and MCG in anoxic, organic-rich sediments. Multivariate analyses identified sulphide and dissolved organic carbon as predictor variables of archaeal community composition. Quantification of MCG using a newly designed qPCR primer pair that improves coverage for MCG subgroups prevalent in the studied lakes revealed conspicuous populations in both the plankton and the sediment. Subgroups MCG-5a and -5b appear as planktonic specialists thriving in euxinic bottom waters, while subgroup MCG-6 emerges as a generalist group able to cope with varying reducing conditions. Besides, comparison of DNA- and cDNA-based pyrotag libraries revealed that rare subgroups in DNA libraries, i.e. MCG-15, were prevalent in cDNA-based datasets, suggesting that euxinic, organic-rich sediments of karstic lakes provide optimal niches for the activity of some specialized MCG subgroups

This research was funded by projects ARCANOX (Ref. CGL2009-13318-C02–02) and ARCOS (ref. CGL2012–33033) from the Spanish Ministry of Economy and Competitiveness (MINECO)

© FEMS Microbiology Ecology, 2015, vol. 91, p. fiv020

Wiley

Manager: Ministerio de Ciencia e Innovación (Espanya)
Ministerio de Economía y Competitividad (Espanya)
Author: Fillol Homs, Mireia
Sànchez Melsió, Alexandre
Gich Batlle, Frederic
Borrego i Moré, Carles
Date: 2015 January
Abstract: The Miscellaneous Crenarchaeotic Group (MCG) is an archaeal lineage whose members are widespread and abundant in marine sediments. MCG archaea have also been consistently found in stratified euxinic lakes. In this work, we have studied archaeal communities in three karstic lakes to reveal potential habitat segregation of MCG subgroups between planktonic and sediment compartments. In the studied lakes, archaeal assemblages were strikingly similar to those of the marine subsurface with predominance of uncultured Halobacteria in the plankton and Thermoplasmata and MCG in anoxic, organic-rich sediments. Multivariate analyses identified sulphide and dissolved organic carbon as predictor variables of archaeal community composition. Quantification of MCG using a newly designed qPCR primer pair that improves coverage for MCG subgroups prevalent in the studied lakes revealed conspicuous populations in both the plankton and the sediment. Subgroups MCG-5a and -5b appear as planktonic specialists thriving in euxinic bottom waters, while subgroup MCG-6 emerges as a generalist group able to cope with varying reducing conditions. Besides, comparison of DNA- and cDNA-based pyrotag libraries revealed that rare subgroups in DNA libraries, i.e. MCG-15, were prevalent in cDNA-based datasets, suggesting that euxinic, organic-rich sediments of karstic lakes provide optimal niches for the activity of some specialized MCG subgroups
This research was funded by projects ARCANOX (Ref. CGL2009-13318-C02–02) and ARCOS (ref. CGL2012–33033) from the Spanish Ministry of Economy and Competitiveness (MINECO)
Format: application/pdf
Citation: 023887
ISSN: 0168-6496 (versió paper)
1574-6941 (versió electrònica)
Document access: http://hdl.handle.net/10256/13408
Language: eng
Publisher: Wiley
Collection: MICINN/PN 2010-2012/CGL2009-13318-C02-02
MINECO/PN 2013-2015/CGL2012-33033
Reproducció digital del document publicat a: http://dx.doi.org/10.1093/femsec/fiv020
Articles publicats (D-B)
Is part of: © FEMS Microbiology Ecology, 2015, vol. 91, p. fiv020
Rights: Tots els drets reservats
Subject: Microbiologia d’aigua dolça
Freshwater microbiology
Microbiologia -- Cultius i medis de cultiu
Microbiology -- Cultures and culture media
Sediments marins
Marine sediments
Title: Diversity of Miscellaneous Crenarchaeotic Group archaea in freshwater karstic lakes and their segregation between planktonic and sediment habitats
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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