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A comparative study of the effects of Escherichia coli and Clostridium perfringens upon boar semen preserved in liquid storage

The present study compares the sperm quality of boar seminal doses artificially inoculated with Escherichia coli and Clostridium perfringens, and maintained in liquid storage at 15 °C for a 9-day period. Seminal doses from 10 sexually mature Piétrain boars were diluted in a Beltsville Thawing Solution (BTS)-based extender and infected either with E. coli or C. perfringens, with bacterial loads ranging from 101 to 107 cfu mL−1. During storage, the changes in sperm quality were determined by assessing pH, sperm viability, sperm motiliy, sperm morphology, sperm agglutination degree, and sperm-bacteria interaction. The infection of seminal doses led to an alkalinization of the medium, which was of higher extend in doses infected with C. perfringens. The effect of contamination on sperm viability and motility relied on bacterial type and load. Therefore, while E. coli was more harmful than C. perfringens in bacterial loads ranging from 101 to 106 cfu mL−1, the detrimental impact of C. perfringens was more apparent than that of E. coli at a bacterial load of 107 cfu mL−1. Despite sperm morphology not being affected by either bacterial type or load, sperm agglutination and sperm-bacteria interaction were characteristic of doses infected with E. coli, and increased concomintantly with bacterial load and along storage period. In conclusion, the effects of infection by E. coli on sperm quality were dependent of both bacterial load and storage period, whereas the effects of C. perfringens were mainly dependent on the bacterial load, with a threshold at 107 cfu mL−1 from which the sperm quality of seminal doses was greatly impaired

The present research was supported by Ministry of Economy and Competitiveness, Spain (Grant code: AGL2013-47798-P)

© Animal Reproduction Science, 2017, vol. 177, p. 65-78

Elsevier

Manager: Ministerio de Economía y Competitividad (Espanya)
Author: Pinart Nadal, Elisabeth
Domènech, Esther
Bussalleu Muntada, Eva
Yeste Oliveras, Marc
Bonet, Sergi
Date: 2017 February 1
Abstract: The present study compares the sperm quality of boar seminal doses artificially inoculated with Escherichia coli and Clostridium perfringens, and maintained in liquid storage at 15 °C for a 9-day period. Seminal doses from 10 sexually mature Piétrain boars were diluted in a Beltsville Thawing Solution (BTS)-based extender and infected either with E. coli or C. perfringens, with bacterial loads ranging from 101 to 107 cfu mL−1. During storage, the changes in sperm quality were determined by assessing pH, sperm viability, sperm motiliy, sperm morphology, sperm agglutination degree, and sperm-bacteria interaction. The infection of seminal doses led to an alkalinization of the medium, which was of higher extend in doses infected with C. perfringens. The effect of contamination on sperm viability and motility relied on bacterial type and load. Therefore, while E. coli was more harmful than C. perfringens in bacterial loads ranging from 101 to 106 cfu mL−1, the detrimental impact of C. perfringens was more apparent than that of E. coli at a bacterial load of 107 cfu mL−1. Despite sperm morphology not being affected by either bacterial type or load, sperm agglutination and sperm-bacteria interaction were characteristic of doses infected with E. coli, and increased concomintantly with bacterial load and along storage period. In conclusion, the effects of infection by E. coli on sperm quality were dependent of both bacterial load and storage period, whereas the effects of C. perfringens were mainly dependent on the bacterial load, with a threshold at 107 cfu mL−1 from which the sperm quality of seminal doses was greatly impaired
The present research was supported by Ministry of Economy and Competitiveness, Spain (Grant code: AGL2013-47798-P)
Format: application/pdf
Citation: 026514
ISSN: 0378-4320 (versió paper)
1873-2232 (versió electrònica)
Document access: http://hdl.handle.net/10256/13720
Language: eng
Publisher: Elsevier
Collection: MINECO/PE 2014-2016/AGL2013-47798-P
Reproducció digital del document publicat a: http://dx.doi.org/10.1016/j.anireprosci.2016.12.007
Articles publicats (D-B)
Is part of: © Animal Reproduction Science, 2017, vol. 177, p. 65-78
Rights: Tots els drets reservats
Subject: Porcs -- Espermatozoides
Swine -- Spermatozoa
Clostridium perfringens
Escheríchia coli
Semen
Porcs -- Inseminació artificial
Swine -- Artificial insemination
Title: A comparative study of the effects of Escherichia coli and Clostridium perfringens upon boar semen preserved in liquid storage
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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