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Sample preservation for the analysis of antibiotics in water

This paper describes a stability study performed for 56 antibiotics belonging to 9 different groups - macrolides, tetracyclines, fluoroquinolones, quinolones, penicillins, cephalosporines, lincosamides, sulfonamides and nitroimidazole antibiotics - in purified water samples fortified with the selected compounds at 10. ng/ml. For this purpose, three different sample preservation modes were tested with the aim of avoiding biotic and abiotic degradation: (i) storage at -20. °C, (ii) storage at -20. °C with 0.1% of EDTA and (iii) pre-concentration in a solid phase extraction cartridge (SPE), which was afterwards stored at -20. °C. Concentrations of antibiotics in the samples preserved using the different protocols were monitored after 0, 1, 2 and 12 weeks. The results showed that, for the accurate determination of all compounds they should be analyzed right after sampling. However, if this is not possible, most of the antibiotics can be analyzed within the 1st week after sampling and preservation at -20. °C (with or without EDTA) or in a SPE cartridges at -20. °C. Nonetheless, some antibiotics found extensively in the environment, such as sulfamethoxazole, ciprofloxacin, ofloxacin, erythromycin, azithromycin and clarithromycin exhibited low stability after 1 week preservation and, therefore, they should be analyzed within this time

© Journal of Chromatography A, 2014, vol. 1369, p. 43-51

Elsevier

Author: Llorca, Marta
Gros, Meritxell
Rodríguez Mozaz, Sara
Barceló i Cullerés, Damià
Date: 2014 November 21
Abstract: This paper describes a stability study performed for 56 antibiotics belonging to 9 different groups - macrolides, tetracyclines, fluoroquinolones, quinolones, penicillins, cephalosporines, lincosamides, sulfonamides and nitroimidazole antibiotics - in purified water samples fortified with the selected compounds at 10. ng/ml. For this purpose, three different sample preservation modes were tested with the aim of avoiding biotic and abiotic degradation: (i) storage at -20. °C, (ii) storage at -20. °C with 0.1% of EDTA and (iii) pre-concentration in a solid phase extraction cartridge (SPE), which was afterwards stored at -20. °C. Concentrations of antibiotics in the samples preserved using the different protocols were monitored after 0, 1, 2 and 12 weeks. The results showed that, for the accurate determination of all compounds they should be analyzed right after sampling. However, if this is not possible, most of the antibiotics can be analyzed within the 1st week after sampling and preservation at -20. °C (with or without EDTA) or in a SPE cartridges at -20. °C. Nonetheless, some antibiotics found extensively in the environment, such as sulfamethoxazole, ciprofloxacin, ofloxacin, erythromycin, azithromycin and clarithromycin exhibited low stability after 1 week preservation and, therefore, they should be analyzed within this time
Format: application/pdf
Citation: https://doi.org/10.1016/j.chroma.2014.09.089
ISSN: 0021-9673
Document access: http://hdl.handle.net/10256/14350
Language: eng
Publisher: Elsevier
Collection: Versió postprint del document publicat a: https://doi.org/10.1016/j.chroma.2014.09.089
Articles publicats (ICRA)
Is part of: © Journal of Chromatography A, 2014, vol. 1369, p. 43-51
Rights: Tots els drets reservats
Subject: Contaminants emergents en l’aigua
Emerging contaminants in water
Cromatografia
Chromatographic analysis
Espectrometria de masses
Mass spectrometry
Aigua -- Contaminació
Water -- Pollution
Medicaments -- Aspectes ambientals
Drugs -- Environmental aspects
Title: Sample preservation for the analysis of antibiotics in water
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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