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Multi-residue method for the determination of antibiotics and some of their metabolites in seafood

El títol de la versió postprint d’aquest article és: ’Fast methodology for the determination of a broad set of antibiotics and some of their metabolites in seafood’

The presence of antibiotics in seafood for human consumption may pose a risk for consumers. A methodology for the analysis of antibiotics in seafood based on QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction, followed by detection and quantification using liquid chromatography coupled to mass spectrometry was developed. The analytical method was evaluated for the determination of 23 antibiotics (including parent compounds and some metabolites) in fish, mussels and clams. Recoveries ranged between 30% and 70% for most of the compounds and method detection and quantification limits (MDLs and MQLs) were between 0.01 and 0.31 ng/g dry weigh (dw) and 0.02–1.03 ng/g (dw) respectively. Real seafood samples were analysed using this method. Nine antibiotics were found at levels above MDLs; however none of them exceed the maximum residue limits (MRL) established by the authorities. Tetracycline was the most ubiquitous compound, presenting also the highest concentration: 5.63 ng/g (dw) in fish from Netherlands. In addition, an alternative technique based on microbial growth inhibition was explored as semiquantitative detection method of antibiotics in seafood. This methodology c ould be applied as a fast screening technique for the detection of macrolides and β-lactams in seafood but further research is needed for other antibiotics families

The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and the SEA-on-a-CHIP project (grant agreement no. 614168). It has been co-financed by the European Union through the European Regional Development Fund (ERDF). This work has also been partly funded by the Generalitat de Catalunya (Consolidated Research Groups 2014-SGR-291-Catalan Institute for Water Research 2014 & 2014-SGR-418-Water and Soil Quality Unit)

© Food and Chemical Toxicology, 2017, vol. 104, p. 3-13

Elsevier

Author: Serra Compte, Albert
Álvarez-Muñoz, Diana
Rodríguez Mozaz, Sara
Barceló i Cullerés, Damià
Date: 2017 June 1
Abstract: El títol de la versió postprint d’aquest article és: ’Fast methodology for the determination of a broad set of antibiotics and some of their metabolites in seafood’
The presence of antibiotics in seafood for human consumption may pose a risk for consumers. A methodology for the analysis of antibiotics in seafood based on QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction, followed by detection and quantification using liquid chromatography coupled to mass spectrometry was developed. The analytical method was evaluated for the determination of 23 antibiotics (including parent compounds and some metabolites) in fish, mussels and clams. Recoveries ranged between 30% and 70% for most of the compounds and method detection and quantification limits (MDLs and MQLs) were between 0.01 and 0.31 ng/g dry weigh (dw) and 0.02–1.03 ng/g (dw) respectively. Real seafood samples were analysed using this method. Nine antibiotics were found at levels above MDLs; however none of them exceed the maximum residue limits (MRL) established by the authorities. Tetracycline was the most ubiquitous compound, presenting also the highest concentration: 5.63 ng/g (dw) in fish from Netherlands. In addition, an alternative technique based on microbial growth inhibition was explored as semiquantitative detection method of antibiotics in seafood. This methodology c ould be applied as a fast screening technique for the detection of macrolides and β-lactams in seafood but further research is needed for other antibiotics families
The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and the SEA-on-a-CHIP project (grant agreement no. 614168). It has been co-financed by the European Union through the European Regional Development Fund (ERDF). This work has also been partly funded by the Generalitat de Catalunya (Consolidated Research Groups 2014-SGR-291-Catalan Institute for Water Research 2014 & 2014-SGR-418-Water and Soil Quality Unit)
Format: application/pdf
Citation: https://doi.org/10.1016/j.fct.2016.11.031
ISSN: 0278-6915
Document access: http://hdl.handle.net/10256/14366
Language: eng
Publisher: Elsevier
Collection: Versió postprint del document publicat a: https://doi.org/10.1016/j.fct.2016.11.031
Articles publicats (ICRA)
info:eu-repo/grantAgreement/EC/FP7/311820
info:eu-repo/grantAgreement/EC/FP7/614168
Is part of: © Food and Chemical Toxicology, 2017, vol. 104, p. 3-13
Rights: Tots els drets reservats
Subject: Antibiòtics
Antibiotics
Cromatografia de líquids
Liquid chromatography
Espectrometria de masses
Mass spectrometry
Contaminants emergents en l’aigua
Emerging contaminants in water
Toxicologia ambiental
Environmental toxicology
Marisc -- Toxicologia
Seafood -- Toxicology
Title: Multi-residue method for the determination of antibiotics and some of their metabolites in seafood
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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