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Exploring Parasitic Load in European Sardine: Applying Two Methodological Approaches along the Catalan Coast

The European sardine, Sardina pilchardus (Walbaum, 1792), is a cold-temperate water species from the Clupeid family. This small pelagic has a key functional role in the marine ecosystem along its distributional range in the northeast of the Atlantic Ocean, the Sea of Marmara, the Black Sea and the Mediterranean Sea. Furthermore, sardine is one of the most important commercial fishery resources caught by the purse seine fleet in the Mediterranean Sea. There is reported a decline in annual sardine catches in the Northwestern Mediterranean Sea, as well as smaller mean total length and sexual maturation size of the individuals, mainly attributed to increased water temperature and overfishing, which have a negative impact on energetic body condition, growth and reproduction. However, little is known about the impact of parasitism on sardine health status. In this work we analyzed the incidence of parasites in sardines from the Catalan Coast of the Northwestern Iberian Peninsula, using two approaches: visual inspection by stereo microscope and the UV-press method, based on the autofluorescence of certain parasites, along a complete reproductive cycle. The main parasite found was the nematode Hysterothylacium sp., although other species were identified by visual inspection (i.e., Contracaecum sp., digenea trematodes). No Anisakis spp. were observed under either of the methodologies. Using the former method, we detected 35.3 % of individuals infected by, at least, one nematode parasite with a mean intensity of 1.68 %. With the UV-press technique, we quantified a prevalence of 16.5 %. In both cases, the Southern Catalan Coast presented a larger number of parasitized sardines, which was remarkably higher in spring. Combined methodologies are suggested to more precisely detect parasites, since visual inspection allows a more detailed study of the viscera, but UV-press also allows the study of parasites that may be present in the musculature. Furthermore, genetic validation should be performed in order to accurately determine the parasite species found

We want to thank the funding support of the Spanish Ministry of Science, Innovation and Universities (RTI2018-097544-B-I00, ‘ConSarVar’, I+D+i Retos de Investigación), as well as the Fons Europeu Marítim i de la Pesca (FEMP) and the Agency for Management of University and Research Grants (AGAUR) of the Generalitat de Catalunya (Ajuts per a la contractació de personal investigador novell (FI-2020))

MDPI (Multidisciplinary Digital Publishing Institute)

Manager: Agencia Estatal de Investigación
Author: Caballero-Huertas, Marta
Frigola-Tepe, Xènia
Muñoz Frigola, Marta
Mattiucci, Simonetta
Viñas de Puig, Jordi
Date: 2022 June 7
Abstract: The European sardine, Sardina pilchardus (Walbaum, 1792), is a cold-temperate water species from the Clupeid family. This small pelagic has a key functional role in the marine ecosystem along its distributional range in the northeast of the Atlantic Ocean, the Sea of Marmara, the Black Sea and the Mediterranean Sea. Furthermore, sardine is one of the most important commercial fishery resources caught by the purse seine fleet in the Mediterranean Sea. There is reported a decline in annual sardine catches in the Northwestern Mediterranean Sea, as well as smaller mean total length and sexual maturation size of the individuals, mainly attributed to increased water temperature and overfishing, which have a negative impact on energetic body condition, growth and reproduction. However, little is known about the impact of parasitism on sardine health status. In this work we analyzed the incidence of parasites in sardines from the Catalan Coast of the Northwestern Iberian Peninsula, using two approaches: visual inspection by stereo microscope and the UV-press method, based on the autofluorescence of certain parasites, along a complete reproductive cycle. The main parasite found was the nematode Hysterothylacium sp., although other species were identified by visual inspection (i.e., Contracaecum sp., digenea trematodes). No Anisakis spp. were observed under either of the methodologies. Using the former method, we detected 35.3 % of individuals infected by, at least, one nematode parasite with a mean intensity of 1.68 %. With the UV-press technique, we quantified a prevalence of 16.5 %. In both cases, the Southern Catalan Coast presented a larger number of parasitized sardines, which was remarkably higher in spring. Combined methodologies are suggested to more precisely detect parasites, since visual inspection allows a more detailed study of the viscera, but UV-press also allows the study of parasites that may be present in the musculature. Furthermore, genetic validation should be performed in order to accurately determine the parasite species found
We want to thank the funding support of the Spanish Ministry of Science, Innovation and Universities (RTI2018-097544-B-I00, ‘ConSarVar’, I+D+i Retos de Investigación), as well as the Fons Europeu Marítim i de la Pesca (FEMP) and the Agency for Management of University and Research Grants (AGAUR) of the Generalitat de Catalunya (Ajuts per a la contractació de personal investigador novell (FI-2020))
Format: application/pdf
Document access: http://hdl.handle.net/10256/21175
Language: eng
Publisher: MDPI (Multidisciplinary Digital Publishing Institute)
Collection: info:eu-repo/semantics/altIdentifier/doi/10.3390/blsf2022013043
info:eu-repo/semantics/altIdentifier/eissn/2673-9976
RTI2018-097544-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-097544-B-I00/ES/ANALISIS DE LA VARIABILIDAD GENOMICA Y CONDICION EN LA POBLACIONES DE SARDINA DEL MEDITERRANEO. IMPLICACION PARA LA GESTION DE LA PESQUERIA/
Rights: Attribution 4.0 International
Rights URI: http://creativecommons.org/licenses/by/4.0/
Subject: Sardines -- Paràsits
Sardines -- Parasites
Sardines -- Reproducció
Sardines -- Reproduction
Genòmica
Genomics
Title: Exploring Parasitic Load in European Sardine: Applying Two Methodological Approaches along the Catalan Coast
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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