DUGi

Perfil de antioxidantes e impacto del líquido folicular preovulatorio sobre el control de las especies reactivas de oxígeno (ROS) en espermatozoides de burro

http://dugi.udg.edu/item/http:@@@@hdl.handle.net@@10256@@26270

Assisted reproduction techniques (ARTs) subject sperm to stressful processes that can cause an increase in the generation of reactive oxygen species (ROS). Because spermatozoa do not have sufficient protection mechanisms to counteract an overproduction of ROS, they end up causing damage to various structures and processes of spermatozoa, decreasing their fertilizing capacity. The follicular fluid (FF) is responsible for providing the follicles with the ideal environment to develop and mature properly. This fluid not only provides nutrients, hormones, and other factors to follicles, but also protects them from oxidative stress. Due to this, there is an interest in checking whether FF could also play this protection against ROS in spermatozoa. This study aimed to determine the capacity of the preovulatory follicular fluid (FFP) of jennies to regulate the oxidative stress of the spermatozoa of jackasses and, therefore, to mitigate the damage it produces in various processes and structures of the spermatozoa. This study made use of nine ejaculates of three Catalan jackasses (three from each jackass) and ten FFP samples of pre-ovulatory follicles of five Catalan jennies (two per jenny). Once the samples were processed the semen samples underwent two treatments; the first consisted only of TBM medium and the second of TBM medium supplemented with FFP at 20 %. Both treatments were incubated for 0, 60, 70 and 120 minutes at a temperature of 38 ºC. In each time 200 µL of sample was extracted and subsequently divided into two aliquots: one of them was used for the analysis of systematically computerized motility CASA and the other to evaluate different parameters of seminal quality by flow cytometry. In conclusion, this study describes for the first time the antioxidant profile, both enzymatic and non-enzymatic antioxidants, of the Catalan jenny’s FFP. It has also been shown that exposure of the spermatozoa to FFP, decreases the intracellular levels of ROS and provides protection against negative effects of oxidative stress, improving viability and sperm motility. Effects that would be related to the antioxidant capacity of FFP

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