Ítem


Multifunctionality and Diversity in Bacterial Biofilms

Bacteria are highly diverse and drive a bulk of ecosystem processes. Analysis of relationships between diversity and single specific ecosystem processes neglects the possibility that different species perform multiple functions at the same time. The degradation of dissolved organic carbon (DOC) followed by respiration is a key bacterial function that is modulated by the availability of DOC and the capability to produce extracellular enzymes. In freshwater ecosystems, biofilms are metabolic hotspots and major sites of DOC degradation. We manipulated the diversity of biofilm forming communities which were fed with DOC differing in availability. We characterized community composition using molecular fingerprinting (T-RFLP) and measured functioning as oxygen consumption rates, the conversion of DOC in the medium, bacterial abundance and the activities of five specific enzymes. Based on assays of the extracellular enzyme activity, we calculated how the likelihood of sustaining multiple functions was affected by reduced diversity. Carbon source and biofilm age were strong drivers of community functioning, and we demonstrate how the likelihood of sustaining multifunctionality decreases with decreasing diversity

PLoS ONE, 2011, vol. 6, núm. 8, p. e23225

Public Library of Science

Autor: Peter, Hannes
Ylla i Monfort, Irene
Gudasz, Cristian
Romaní i Cornet, Anna M.
Sabater, Sergi
Tranvik, Lars J.
Data: agost 2011
Resum: Bacteria are highly diverse and drive a bulk of ecosystem processes. Analysis of relationships between diversity and single specific ecosystem processes neglects the possibility that different species perform multiple functions at the same time. The degradation of dissolved organic carbon (DOC) followed by respiration is a key bacterial function that is modulated by the availability of DOC and the capability to produce extracellular enzymes. In freshwater ecosystems, biofilms are metabolic hotspots and major sites of DOC degradation. We manipulated the diversity of biofilm forming communities which were fed with DOC differing in availability. We characterized community composition using molecular fingerprinting (T-RFLP) and measured functioning as oxygen consumption rates, the conversion of DOC in the medium, bacterial abundance and the activities of five specific enzymes. Based on assays of the extracellular enzyme activity, we calculated how the likelihood of sustaining multiple functions was affected by reduced diversity. Carbon source and biofilm age were strong drivers of community functioning, and we demonstrate how the likelihood of sustaining multifunctionality decreases with decreasing diversity
Format: application/pdf
Cita: https://doi.org/10.1371/journal.pone.0023225
ISSN: 1932-6203
Accés al document: http://hdl.handle.net/10256/7791
Llenguatge: eng
Editor: Public Library of Science
Col·lecció: Reproducció digital del document publicat a: http://dx.doi.org/10.1371/ journal.pone.0023225
Articles publicats (D-CCAA)
És part de: PLoS ONE, 2011, vol. 6, núm. 8, p. e23225
Drets: Attribution 3.0 Spain
URI Drets: http://creativecommons.org/licenses/by/3.0/es/
Matèria: Biofilms
Bacteris
Bacteria
Títol: Multifunctionality and Diversity in Bacterial Biofilms
Tipus: info:eu-repo/semantics/article
Repositori: DUGiDocs

Matèries

Autors