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Evaluation of potential breath biomarkers for active smoking: assessment of smoking habits

Different compounds have been reported as biomarkers of a smoking habit, but, to date, there is no appropriate biomarker for tobacco-related exposure because the proposed chemicals seem to be nonspecific or they are only appropriate for short-term exposure. Moreover, conventional sampling methodologies require an invasive method because blood or urine samples are required. The use of a microtrap system coupled to gas chromatography–mass spectrometry analysis has been found to be very effective for the noninvasive analysis of volatile organic compounds in breath samples. The levels of benzene, 2,5-dimethylfuran, toluene, o-xylene, and m- p-xylene have been analyzed in breath samples obtained from 204 volunteers (100 smokers, 104 nonsmokers; 147 females, 57 males; ages 16 to 53 years). 2,5-Dimethylfuran was always below the limit of detection (0.005 ppbv) in the nonsmoker population and always detected in smokers independently of the smoking habits. Benzene was only an effective biomarker for medium and heavy smokers, and its level was affected by smoking habits. Regarding the levels of xylenes and toluene, they were only different in heavy smokers and after short-term exposure. The results obtained suggest that 2,5-dimethylfuran is a specific breath biomarker of smoking status independently of the smoking habits (e.g., short- and long-term exposure, light and heavy consumption), and so this compound might be useful as a biomarker of smoking exposure

The final publication is available at link.springer.com

© Analytical and Bioanalytical Chemistry, vol. 396, núm. 8, p. 2987-2995

Springer

Author: Alonso Roura, Mònica
Castellanos, María del Mar
Sánchez Navarro, Juan Manuel
Date: 2010 April
Abstract: Different compounds have been reported as biomarkers of a smoking habit, but, to date, there is no appropriate biomarker for tobacco-related exposure because the proposed chemicals seem to be nonspecific or they are only appropriate for short-term exposure. Moreover, conventional sampling methodologies require an invasive method because blood or urine samples are required. The use of a microtrap system coupled to gas chromatography–mass spectrometry analysis has been found to be very effective for the noninvasive analysis of volatile organic compounds in breath samples. The levels of benzene, 2,5-dimethylfuran, toluene, o-xylene, and m- p-xylene have been analyzed in breath samples obtained from 204 volunteers (100 smokers, 104 nonsmokers; 147 females, 57 males; ages 16 to 53 years). 2,5-Dimethylfuran was always below the limit of detection (0.005 ppbv) in the nonsmoker population and always detected in smokers independently of the smoking habits. Benzene was only an effective biomarker for medium and heavy smokers, and its level was affected by smoking habits. Regarding the levels of xylenes and toluene, they were only different in heavy smokers and after short-term exposure. The results obtained suggest that 2,5-dimethylfuran is a specific breath biomarker of smoking status independently of the smoking habits (e.g., short- and long-term exposure, light and heavy consumption), and so this compound might be useful as a biomarker of smoking exposure
The final publication is available at link.springer.com
Format: application/pdf
ISSN: 1618-2642 (versió paper)
1618-2650 (versió electrònica)
Document access: http://hdl.handle.net/10256/7863
Language: eng
Publisher: Springer
Collection: Versió postprint del document publicat a: http://dx.doi.org/10.1007/s00216-010-3524-z
Articles publicats (D-Q)
Is part of: © Analytical and Bioanalytical Chemistry, vol. 396, núm. 8, p. 2987-2995
Rights: Tots els drets reservats
Subject: Hàbit de fumar
Smoking
Fumadors
Cigarette smokers
Benzè
Benzene
Compostos heterocíclics
Heterocyclic compounds
Furans
Marcadors bioquímics
Biochemical markers
Title: Evaluation of potential breath biomarkers for active smoking: assessment of smoking habits
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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