Warning: session_start() [function.session-start]: open(/var/lib/php5/sess_18c695be99aaac3482982f9461bcddbc, O_RDWR) failed: Read-only file system (30) in /dades/dugi/start_cache.php on line 4

Warning: session_start() [function.session-start]: Cannot send session cookie - headers already sent by (output started at /dades/dugi/start_cache.php:4) in /dades/dugi/start_cache.php on line 4

Warning: session_start() [function.session-start]: Cannot send session cache limiter - headers already sent (output started at /dades/dugi/start_cache.php:4) in /dades/dugi/start_cache.php on line 4

Warning: Cannot modify header information - headers already sent by (output started at /dades/dugi/start_cache.php:4) in /dades/dugi/start_cache.php on line 7

Warning: error_log(/dades/dugi/log//querys.log) [function.error-log]: failed to open stream: Read-only file system in /dades/dugi/lib/log/log.php on line 32
DUGi: Ítem | Recercat - Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC)

Ítem


Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC)

Crohn’s disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a feature of certain pathogenic E. coli strains, we compared the biofilmformation capacity of 27 AIEC and 38 non-AIEC strains isolated from the intestinal mucosa. Biofilmformation capacity was then contrasted with the AIEC phenotype, the serotype, the phylotype, andthe presence of virulence genes. Results: Specific biofilm formation (SBF) indices were higher amongst AIEC than non-AIEC strains(P = 0.012). In addition, 65.4% of moderate to strong biofilms producers were AIEC, whereas74.4% of weak biofilm producers were non-AIEC (P = 0.002). These data indicate that AIEC strainswere more efficient biofilm producers than non-AIEC strains. Moreover, adhesion (P = 0.009) andinvasion (P = 0.003) indices correlated positively with higher SBF indices. Additionally, motility(100%, P < 0.001), H1 type flagellin (53.8%, P < 0.001), serogroups O83 (19.2%, P = 0.008) and O22(26.9%, P = 0.001), the presence of virulence genes such as sfa/focDE (38.5%, P = 0.003) and ibeA(26.9%, P = 0.017), and B2 phylotype (80.8%, P < 0.001) were frequent characteristics amongstbiofilm producers.Conclusion: The principal contribution of the present work is the finding that biofilm formationcapacity is a novel, complementary pathogenic feature of the recently described AIEC pathovar. Characterization of AIEC specific genetic determinants, and the regulatory pathways, involved in biofilm formation will likely bring new insights into AIEC pathogenesis

BioMed Central

Autor: Martínez Medina, Margarita
Naves, Plínio
Blanco, Jorge
Aldeguer, Xavier
Blanco, Jesús E.
Blanco, Miguel
Ponte, Carmen
Soriano, Francisco
Darfeuille-Michaud, Arlette
Garcia-Gil, L. J.
Resum: Crohn’s disease (CD) is a high morbidity chronic inflammatory disorder of unknown aetiology. Adherent-invasive Escherichia coli (AIEC) has been recently implicated in the origin and perpetuation of CD. Because bacterial biofilms in the gut mucosa are suspected to play a role in CD and biofilm formation is a feature of certain pathogenic E. coli strains, we compared the biofilmformation capacity of 27 AIEC and 38 non-AIEC strains isolated from the intestinal mucosa. Biofilmformation capacity was then contrasted with the AIEC phenotype, the serotype, the phylotype, andthe presence of virulence genes. Results: Specific biofilm formation (SBF) indices were higher amongst AIEC than non-AIEC strains(P = 0.012). In addition, 65.4% of moderate to strong biofilms producers were AIEC, whereas74.4% of weak biofilm producers were non-AIEC (P = 0.002). These data indicate that AIEC strainswere more efficient biofilm producers than non-AIEC strains. Moreover, adhesion (P = 0.009) andinvasion (P = 0.003) indices correlated positively with higher SBF indices. Additionally, motility(100%, P < 0.001), H1 type flagellin (53.8%, P < 0.001), serogroups O83 (19.2%, P = 0.008) and O22(26.9%, P = 0.001), the presence of virulence genes such as sfa/focDE (38.5%, P = 0.003) and ibeA(26.9%, P = 0.017), and B2 phylotype (80.8%, P < 0.001) were frequent characteristics amongstbiofilm producers.Conclusion: The principal contribution of the present work is the finding that biofilm formationcapacity is a novel, complementary pathogenic feature of the recently described AIEC pathovar. Characterization of AIEC specific genetic determinants, and the regulatory pathways, involved in biofilm formation will likely bring new insights into AIEC pathogenesis
Accés al document: http://hdl.handle.net/2072/180265
Llenguatge: eng
Editor: BioMed Central
Drets: Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
URI Drets: http://creativecommons.org/licenses/by/2.0/es/deed.ca
Matèria: Crohn, Malaltia de
Infeccions per escheríchia coli
Crohn’s disease
Escherichia coli infections
Títol: Biofilm formation as a novel phenotypic feature of adherent-invasive Escherichia coli (AIEC)
Tipus: info:eu-repo/semantics/article
Repositori: Recercat

Matèries


Warning: error_log(/dades/dugi/log//dugi.log) [function.error-log]: failed to open stream: Read-only file system in /dades/dugi/lib/log/log.php on line 32

Autors


Warning: error_log(/dades/dugi/log//dugi.log) [function.error-log]: failed to open stream: Read-only file system in /dades/dugi/lib/log/log.php on line 32


Warning: fopen(/dades/dugi/cache/170e10a2b246c0c20413b0a16cc4a7c8_.html) [function.fopen]: failed to open stream: Read-only file system in /dades/dugi/end_cache.php on line 2

Warning: Unknown: open(/var/lib/php5/sess_18c695be99aaac3482982f9461bcddbc, O_RDWR) failed: Read-only file system (30) in Unknown on line 0

Warning: Unknown: Failed to write session data (files). Please verify that the current setting of session.save_path is correct (/var/lib/php5) in Unknown on line 0