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Lectin histochemistry of the boar bulbourethral glands

The present study describes, for the first time, the glycosidic content of boar bulbourethral glands using lectin histochemistry. Fourteen horseradish peroxidase- or digoxigeninlabelled lectins with different carbohydrate specificities were used in samples obtained from 3 healthy Landrace boars. The results obtained indicate that endpiece and duct cells synthesize and secrete mainly O-glycoproteins with α- and β-D-N-acetylgalactosamine, β -D-galactose-b(1→ 3)-D-Nacetylgalactosamine, D-N-acetylglucosamine and neuraminic acid residues. Glycoproteins secreted by bulbourethral glands have a role in the protection and lubrication of the urethra. In addition, they may be also involved in the regulation of the sperm metabolic activity and in the maintenance of the structural integrity of acrosomal and plasma membranes

This work was supported in part by grant FPUAP99 40325284 awarded to E.Badia.This research was further supported by projects AGL2002-01924,RZ02-013 and RZ03-29 with funds provided by the “Ministerio de Ciencia y Tecnología”-MCYT (Spanish Government), and by project 2001SGR-00294 from the “Direcció General de Recerca” (Autonomous Government of Catalonia)

PAGEPress

Author: Badia Brea, Maria Elena
Pinart Nadal, Elisabeth
Briz González, Maria Dolors
Pastor, L.M.
Sancho Badell, Sílvia
Garcia Gil, Núria
Bassols Casadevall, Judit
Kádár García, Elisabeth
Pruneda Sais, Anna
Bussalleu Muntada, Eva
Yeste Oliveras, Marc
Bonet, Sergi
Abstract: The present study describes, for the first time, the glycosidic content of boar bulbourethral glands using lectin histochemistry. Fourteen horseradish peroxidase- or digoxigeninlabelled lectins with different carbohydrate specificities were used in samples obtained from 3 healthy Landrace boars. The results obtained indicate that endpiece and duct cells synthesize and secrete mainly O-glycoproteins with α- and β-D-N-acetylgalactosamine, β -D-galactose-b(1→ 3)-D-Nacetylgalactosamine, D-N-acetylglucosamine and neuraminic acid residues. Glycoproteins secreted by bulbourethral glands have a role in the protection and lubrication of the urethra. In addition, they may be also involved in the regulation of the sperm metabolic activity and in the maintenance of the structural integrity of acrosomal and plasma membranes
This work was supported in part by grant FPUAP99 40325284 awarded to E.Badia.This research was further supported by projects AGL2002-01924,RZ02-013 and RZ03-29 with funds provided by the “Ministerio de Ciencia y Tecnología”-MCYT (Spanish Government), and by project 2001SGR-00294 from the “Direcció General de Recerca” (Autonomous Government of Catalonia)
Document access: http://hdl.handle.net/2072/217890
Language: eng
Publisher: PAGEPress
Rights: Attribution-NonCommercial 3.0 Spain
Rights URI: http://creativecommons.org/licenses/by-nc/3.0/es/
Subject: Lectines
Lectins
Histoquímica
Histochemistry
Porcs
Pigs
Title: Lectin histochemistry of the boar bulbourethral glands
Type: info:eu-repo/semantics/article
Repository: Recercat

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