Item


Postinfection activity of synthetic antimicrobial peptides against Stemphylium vesicarium in pear

Brown spot of pear is a fungal disease of economic importance caused by Stemphylium vesicarium that affects the pear crops in Europe. Due to the characteristics of this disease and the moderate efficacy of available fungicides, the effectiveness of control measures is very limited; however, synthetic antimicrobial peptides (AMPs) may be a complement to these fungicides. In the present study, 12 AMPs of the CECMEL11 library were screened for fungicidal activity against S. vesicarium. In vitro experiments showed that eight AMPs significantly reduced the germination of conidia. The most effective peptides, BP15, BP22, and BP25, reduced fungal growth and sporulation at concentrations below 50 μM. Leaf assays showed that preventive application of BP15 and BP22 did not reduce infection; however, when the peptides were applied curatively, infection was significantly reduced. The use of a BP15 fluorescein 5-isothiocyanate conjugate revealed that the peptide binds to hyphae and germ tubes and produces malformations that irreversibly stop their development

This research was supported, in part, by grants from the Ministerio de Educacion y Ciencia (AGL2009-09829/AGR) of Spain and the Comissio Interdepartamental de Recerca i Tecnologia of the Generalitat de Catalunya (2009SGR00812). M. Puig was the recipient of a predoctoral fellowship from Universitat de Girona (BR10-17)

American Phytopathological Society

Manager: Ministerio de Ciencia e Innovación (Espanya)
Generalitat de Catalunya. Agència de Gestió d’Ajuts Universitaris i de Recerca
Author: Puig Garcia, Mireia
Moragrega i Garcia, Concepció
Ruz Estévez, Lídia
Montesinos Seguí, Emilio
Llorente i Cabratosa, Isidre
Abstract: Brown spot of pear is a fungal disease of economic importance caused by Stemphylium vesicarium that affects the pear crops in Europe. Due to the characteristics of this disease and the moderate efficacy of available fungicides, the effectiveness of control measures is very limited; however, synthetic antimicrobial peptides (AMPs) may be a complement to these fungicides. In the present study, 12 AMPs of the CECMEL11 library were screened for fungicidal activity against S. vesicarium. In vitro experiments showed that eight AMPs significantly reduced the germination of conidia. The most effective peptides, BP15, BP22, and BP25, reduced fungal growth and sporulation at concentrations below 50 μM. Leaf assays showed that preventive application of BP15 and BP22 did not reduce infection; however, when the peptides were applied curatively, infection was significantly reduced. The use of a BP15 fluorescein 5-isothiocyanate conjugate revealed that the peptide binds to hyphae and germ tubes and produces malformations that irreversibly stop their development
This research was supported, in part, by grants from the Ministerio de Educacion y Ciencia (AGL2009-09829/AGR) of Spain and the Comissio Interdepartamental de Recerca i Tecnologia of the Generalitat de Catalunya (2009SGR00812). M. Puig was the recipient of a predoctoral fellowship from Universitat de Girona (BR10-17)
Document access: http://hdl.handle.net/2072/296851
Language: eng
Publisher: American Phytopathological Society
Rights: Tots els drets reservats
Subject: Fungicides
Fongs en l’agricultura
Fungi in agriculture
Perera -- Malalties i plagues
Pear -- Diseases and pests
Title: Postinfection activity of synthetic antimicrobial peptides against Stemphylium vesicarium in pear
Type: info:eu-repo/semantics/article
Repository: Recercat

Subjects

Authors