Ítem
Ministerio de Ciencia e Innovación (Espanya) | |
Bussalleu Muntada, Eva
Pinart Nadal, Elisabeth Yeste Oliveras, Marc Briz González, Maria Dolors Sancho Badell, Sílvia Torner Bosch, Eva Bonet, Sergi |
|
In semen, bacteria’s isolation from a pure culture is complex, laborious and easily alterable by the presence of antibiotics and inhibitors. We developed a PCR technique to detect the presence of the enterotoxigenic (ETEC) and verotoxigenic Escherichia coli (VTEC) (strains with high prevalence in the swine industry) in semen by adapting the protocols developed by Zhang et al. (2007) and Yilmaz et al. (2006). We artificially inoculated extended semen samples at different infective concentrations of bacteria (from 10 2 to 10 8 bacteriaml -1) with two enterotoxigenic and verotoxigenic strains, and performed two multiplex and one conventional PCR. This technique proved to be a quick, useful and reliable tool to detect the presence of ETEC and VTEC up to an infective dose of 10 5 bacteriaml -1 in semen This research was supported by Project RZ2008-0001-00-00, funds from the ‘‘Ministerio de Ciencia e Innovación’’ (Spanish Government) |
|
http://hdl.handle.net/2072/297726 | |
eng | |
Elsevier | |
Tots els drets reservats | |
Reacció en cadena de la polimerasa
Polymerase chain reaction Porcs -- Espermatozoides Swine -- Spermatozoa Infeccions per escheríchia coli Escherichia coli infections |
|
A PCR technique to detect enterotoxigenic and verotoxigenic Escherichia coli in boar semen samples | |
info:eu-repo/semantics/article | |
Recercat |