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Comparative analysis of prostate-specific antigen by two-dimensional gel electrophoresis and capillary electrophoresis

Serum levels of Prostate-Specific Antigen (PSA) are not fully specific for prostate cancer (PCa) diagnosis and several efforts are focused on searching to improve PCa markers through the study of PSA subforms that could be cancer associated. We have previously reported by 2DE a decrease in the sialic acid content of PSA from PCa compared to benign prostatic hyperplasia patients based on the different proportion of the PSA spots. However, faster and more quantitative techniques, easier to automate than 2DE, are desirable. In this study, we examined the potential of CE for resolving PSA subforms in different samples and compared the results with those obtained by 2DE. We first fractionated by OFFGEL the subforms of PSA from seminal plasma according to their pIs and analyzed each separated fraction by 2DE and CE. We also analyzed PSA and high pI PSA, both from seminal plasma, and PSA from urine of a PCa patient. These samples with different PSA spots proportions by 2DE, due to different posttranslational modifications, also presented different CE profiles. This study shows that CE is a useful and complementary technique to 2DE for analyzing samples with different PSA subforms, which is of high clinical interest

This work was supported by the Spanish Ministerio de Economía y Competitividad (Grant Nos. BIO 2010–16922, BIO2015-66356-R and 2015-66356-R, CTQ 2013-43236-R and CDTI Grant No. IDI20130186) and by the Generalitat de Catalunya, Spain (Grant No. 2014 SGR 229)

Wiley-VCH Verlag

Manager: Ministerio de Ciencia e Innovación (Espanya)
Ministerio de Economía y Competitividad (Espanya)
Author: Barrabés Vera, Sílvia
Farina-Gomez, Noemi
Llop Escorihuela, Esther
Puerta, Angel
Diez-Masa, Jose Carlos
Perry, Antoinette
Llorens Duran, Rafael de
Frutos, Mercedes de
Peracaula Miró, Rosa
Abstract: Serum levels of Prostate-Specific Antigen (PSA) are not fully specific for prostate cancer (PCa) diagnosis and several efforts are focused on searching to improve PCa markers through the study of PSA subforms that could be cancer associated. We have previously reported by 2DE a decrease in the sialic acid content of PSA from PCa compared to benign prostatic hyperplasia patients based on the different proportion of the PSA spots. However, faster and more quantitative techniques, easier to automate than 2DE, are desirable. In this study, we examined the potential of CE for resolving PSA subforms in different samples and compared the results with those obtained by 2DE. We first fractionated by OFFGEL the subforms of PSA from seminal plasma according to their pIs and analyzed each separated fraction by 2DE and CE. We also analyzed PSA and high pI PSA, both from seminal plasma, and PSA from urine of a PCa patient. These samples with different PSA spots proportions by 2DE, due to different posttranslational modifications, also presented different CE profiles. This study shows that CE is a useful and complementary technique to 2DE for analyzing samples with different PSA subforms, which is of high clinical interest
This work was supported by the Spanish Ministerio de Economía y Competitividad (Grant Nos. BIO 2010–16922, BIO2015-66356-R and 2015-66356-R, CTQ 2013-43236-R and CDTI Grant No. IDI20130186) and by the Generalitat de Catalunya, Spain (Grant No. 2014 SGR 229)
Document access: http://hdl.handle.net/2072/299514
Language: eng
Publisher: Wiley-VCH Verlag
Rights: Tots els drets reservats
Subject: Electroforesi capil·lar
Capillary electrophoresis
Pròstata -- Càncer
Prostate -- Cancer
Antígens
Antigens
Immunoelectroforesi bidimensional
Two-dimensional electrophoresis
Title: Comparative analysis of prostate-specific antigen by two-dimensional gel electrophoresis and capillary electrophoresis
Type: info:eu-repo/semantics/article
Repository: Recercat

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