Ítem


Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards

Stemphylium vesicarium is the causal agent of brown spot of pear, an important disease reported in pear-growing areas of Europe. The pathogen is able to colonize pear leaf debris and dead tissues of herbaceous plants and produce abundant ascospores and conidia that are capable of infecting pear trees. Inoculum monitoring in pear orchards is mainly achieved through spore traps and species identification is based on conidial morphology, but the similarities on conidial traits among species of Stemphylium make correct identification difficult. In this work a total of thirty-seven Stemphylium isolates from pear orchards were characterized at the morphological, pathogenic, and molecular level. Correspondence among ITS and gpd sequences and morphological traits were evaluated. Species identification based exclusively on morphological data was not feasible. Combined morphological and molecular data were necessary for unambiguous identification of isolates in the S. vesicarium species group. Only isolates identified as S. vesicarium were pathogenic on pear. The study revealed that several species of Stemphylium coexist in pear orchards with S. vesicarium, the causal agent of BSP, and that combined morphological and molecular data are needed to differentiate them. Consequently, direct measurements of the airborne inoculum using volumetric spore traps may overestimate the actual pathogen population

This research was supported in part by grants from Ministerio de Educacion y Ciencia (AGL2006-04987/AGR and AGL2009-09829/AGR) of Spain; Comissio Interdepartamental de Recerca i Tecnologia from the Generalitat de Catalunya (2009SGR00812) and BR 10/17 from the University of Girona

Elsevier

Director: Ministerio de Educación y Ciencia (Espanya)
Ministerio de Ciencia e Innovación (Espanya)
Autor: Puig Garcia, Mireia
Ruz Estévez, Lídia
Montesinos Seguí, Emilio ​
Moragrega i Garcia, Concepció
Llorente i Cabratosa, Isidre
Data: 5 juny 2018
Resum: Stemphylium vesicarium is the causal agent of brown spot of pear, an important disease reported in pear-growing areas of Europe. The pathogen is able to colonize pear leaf debris and dead tissues of herbaceous plants and produce abundant ascospores and conidia that are capable of infecting pear trees. Inoculum monitoring in pear orchards is mainly achieved through spore traps and species identification is based on conidial morphology, but the similarities on conidial traits among species of Stemphylium make correct identification difficult. In this work a total of thirty-seven Stemphylium isolates from pear orchards were characterized at the morphological, pathogenic, and molecular level. Correspondence among ITS and gpd sequences and morphological traits were evaluated. Species identification based exclusively on morphological data was not feasible. Combined morphological and molecular data were necessary for unambiguous identification of isolates in the S. vesicarium species group. Only isolates identified as S. vesicarium were pathogenic on pear. The study revealed that several species of Stemphylium coexist in pear orchards with S. vesicarium, the causal agent of BSP, and that combined morphological and molecular data are needed to differentiate them. Consequently, direct measurements of the airborne inoculum using volumetric spore traps may overestimate the actual pathogen population
This research was supported in part by grants from Ministerio de Educacion y Ciencia (AGL2006-04987/AGR and AGL2009-09829/AGR) of Spain; Comissio Interdepartamental de Recerca i Tecnologia from the Generalitat de Catalunya (2009SGR00812) and BR 10/17 from the University of Girona
Accés al document: http://hdl.handle.net/2072/319899
Llenguatge: eng
Editor: Elsevier
Drets: Tots els drets reservats
Matèria: Perera -- Malalties i plagues
Pear -- Diseases and pests
Fongs patògens
Pathogenic fungi
Títol: Combined morphological and molecular approach for identification of Stemphylium vesicarium inoculum in pear orchards
Tipus: info:eu-repo/semantics/article
Repositori: Recercat

Matèries

Autors