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A new twist in the photophysics of the GFP chromophore: a volume-conserving molecular torsion couple

The simple structure of the chromophore of the green fluorescent protein (GFP), a phenol and an imidazolone ring linked by a methyne bridge, supports an exceptionally diverse range of excited state phenomena. Here we describe experimentally and theoretically the photochemistry of a novel sterically crowded nonplanar derivative of the GFP chromophore. It undergoes an excited state isomerization reaction accompanied by an exceptionally fast (sub 100 fs) excited state decay. The decay dynamics are essentially independent of solvent polarity and viscosity. Excited state structural dynamics are probed by high level quantum chemical calculations revealing that the fast decay is due to a conical intersection characterized by a twist of the rings and pyramidalization of the methyne bridge carbon. The intersection can be accessed without a barrier from the pre-twisted Franck-Condon structure, and the lack of viscosity dependence is due to the fact that the rings twist in the same direction, giving rise to a volume-conserving decay coordinate. Moreover, the rotation of the phenyl, methyl and imidazolone groups is coupled in the sterically crowded structure, with the methyl group translating the rotation of one ring to the next. As a consequence, the excited state dynamics can be viewed as a torsional couple, where the absorbed photon energy leads to conversion of the out-of-plane orientation from one ring to the other in a volume conserving fashion. A similar modification of the range of methyne dyes may provide a new family of devices for molecular machines, specifically torsional couples

LB acknowledges financial support from Spanish Ministerio deEconomía y Competitividad (UNGI10-4E-801, CTQ2015-69363-P), Departament d’Innovació, Universitats i Empresa (DIUE) from Generalitat de Catalunya (2014SGR-1202), and computational time from Consorci de Serveis Universitaris de Catalunya. SRM is grateful to EPSRC for financial support (EP/M001997/01)

Royal Society of Chemistry (RSC)

Manager: Ministerio de Economía y Competitividad (Espanya)
Author: Conyard, Jamie
Heisler, Ismael A.
Chan, Yohan
Page, Philip C. Bulman
Meech, Stephen R.
Blancafort San José, Lluís
Date: 2020 February 15
Abstract: The simple structure of the chromophore of the green fluorescent protein (GFP), a phenol and an imidazolone ring linked by a methyne bridge, supports an exceptionally diverse range of excited state phenomena. Here we describe experimentally and theoretically the photochemistry of a novel sterically crowded nonplanar derivative of the GFP chromophore. It undergoes an excited state isomerization reaction accompanied by an exceptionally fast (sub 100 fs) excited state decay. The decay dynamics are essentially independent of solvent polarity and viscosity. Excited state structural dynamics are probed by high level quantum chemical calculations revealing that the fast decay is due to a conical intersection characterized by a twist of the rings and pyramidalization of the methyne bridge carbon. The intersection can be accessed without a barrier from the pre-twisted Franck-Condon structure, and the lack of viscosity dependence is due to the fact that the rings twist in the same direction, giving rise to a volume-conserving decay coordinate. Moreover, the rotation of the phenyl, methyl and imidazolone groups is coupled in the sterically crowded structure, with the methyl group translating the rotation of one ring to the next. As a consequence, the excited state dynamics can be viewed as a torsional couple, where the absorbed photon energy leads to conversion of the out-of-plane orientation from one ring to the other in a volume conserving fashion. A similar modification of the range of methyne dyes may provide a new family of devices for molecular machines, specifically torsional couples
LB acknowledges financial support from Spanish Ministerio deEconomía y Competitividad (UNGI10-4E-801, CTQ2015-69363-P), Departament d’Innovació, Universitats i Empresa (DIUE) from Generalitat de Catalunya (2014SGR-1202), and computational time from Consorci de Serveis Universitaris de Catalunya. SRM is grateful to EPSRC for financial support (EP/M001997/01)
Document access: http://hdl.handle.net/2072/373420
Language: eng
Publisher: Royal Society of Chemistry (RSC)
Rights: Reconeixement 4.0 Internacional
Rights URI: http://creativecommons.org/licenses/by/4.0
Subject: Fotoquímica
Photochemistry
Fotoisomerització
Photoisomerization
Title: A new twist in the photophysics of the GFP chromophore: a volume-conserving molecular torsion couple
Type: info:eu-repo/semantics/article
Repository: Recercat

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