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Silencing of the potato StNAC103 gene enhances the accumulation of suberin polyester and associated wax in tuber skin

Suberin and wax deposited in the cork (phellem) layer of the periderm form the lipophilic barrier that protects mature plant organs. Periderm lipids have been widely studied for their protective function with regards to dehydration and for how they respond to environmental stresses and wounding. However, despite advances in the biosynthetic pathways of suberin and associated wax, little is known about the regulation of their deposition. Here, we report on a potato NAC transcription factor gene, StNAC103, induced in the tuber phellem (skin). The StNAC103 promoter is active in cells undergoing suberization such as in the basal layer of the phellem, but also in the root apical meristem. Gene silencing in potato periderm correlates with an increase in the suberin and wax load, and specifically in alkanes, ω-hydroxyacids, diacids, ferulic acid, and primary alcohols. Concomitantly, silenced lines also showed up-regulation of key genes related to the biosynthesis and transport of suberin and wax in the tuber periderm. Taken together, our results suggest that StNAC103 has a role in the tight regulation of the formation of apoplastic barriers and is, to the best of our knowledge, the first candidate gene to be identified as being involved in the repression of suberin and wax deposition

This work was supported by the Ministerio de Educación y Ciencia (AGL2006-07342, FPI grant to OS), the Ministerio de Innovación y Ciencia (AGL2009-13745), the Ministerio de Economía y Competitividad (AGL2012-36725, AGL2015-67495-C2-1-R), Departament d’Universitats Investigació i Societat de la Informació of Catalonia (PhD grants to RV and MS), and the University of Girona (PhD grant to DCA and SF, and grant SING11/1)

Journal of Experimental Botany, 2016,

Oxford University Press (OUP)

Author: Verdaguer, Roger
Soler del Monte, Marçal
Serra i Figueras, Olga
Garrote, Aïda
Fernández Piñan, Sandra
Company Arumí, Dolors
Anticó i Daró, Ma. Enriqueta
Molinas de Ferrer, Maria Lluïsa
Mercè Figueras.
Date: 2016 August 12
Abstract: Suberin and wax deposited in the cork (phellem) layer of the periderm form the lipophilic barrier that protects mature plant organs. Periderm lipids have been widely studied for their protective function with regards to dehydration and for how they respond to environmental stresses and wounding. However, despite advances in the biosynthetic pathways of suberin and associated wax, little is known about the regulation of their deposition. Here, we report on a potato NAC transcription factor gene, StNAC103, induced in the tuber phellem (skin). The StNAC103 promoter is active in cells undergoing suberization such as in the basal layer of the phellem, but also in the root apical meristem. Gene silencing in potato periderm correlates with an increase in the suberin and wax load, and specifically in alkanes, ω-hydroxyacids, diacids, ferulic acid, and primary alcohols. Concomitantly, silenced lines also showed up-regulation of key genes related to the biosynthesis and transport of suberin and wax in the tuber periderm. Taken together, our results suggest that StNAC103 has a role in the tight regulation of the formation of apoplastic barriers and is, to the best of our knowledge, the first candidate gene to be identified as being involved in the repression of suberin and wax deposition
This work was supported by the Ministerio de Educación y Ciencia (AGL2006-07342, FPI grant to OS), the Ministerio de Innovación y Ciencia (AGL2009-13745), the Ministerio de Economía y Competitividad (AGL2012-36725, AGL2015-67495-C2-1-R), Departament d’Universitats Investigació i Societat de la Informació of Catalonia (PhD grants to RV and MS), and the University of Girona (PhD grant to DCA and SF, and grant SING11/1)
Format: application/pdf
ISSN: 0022-0957 (versió paper)
1460-2431 (versió electrònica)
Document access: http://hdl.handle.net/10256/12712
Language: eng
Publisher: Oxford University Press (OUP)
Collection: MEC/PN 2006-2009/AGL2006-07342/AGR
MICINN/PN 2010-2012/AGL2009-13745
MINECO/PN 2013-2015/AGL2012-36725
MINECO/PE 2015-2018/AGL2015-67495-C2-1-R
Reproducció digital del document publicat a: http://dx.doi.org/10.1093/jxb/erw305
Articles publicats (D-B)
Is part of: Journal of Experimental Botany, 2016,
Rights: Reconeixement 3.0 Espanya
Rights URI: http://creativecommons.org/licenses/by/3.0/es/deed.ca
Subject: Biosíntesi
Biosynthesis
Suro
Cork
Title: Silencing of the potato StNAC103 gene enhances the accumulation of suberin polyester and associated wax in tuber skin
Type: info:eu-repo/semantics/article
Repository: DUGiDocs

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